Profiling of volatile organic compounds from Irpex laceratus: An endophytic fungus isolated from Glaucium fimbrilligerum

Document Type : Original Article

Authors

1 Department of Agriculture, Go.C., Islamic Azad University, Gorgan, Iran

2 Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Golestan, Iran

3 Department of Phytopathology, Federal University of Lavras, Minas Gerais, Brazil

4 Department of Horticultural Science, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Golestan, Iran

5 Department of Applied Botany, Federal University of Lavras, Minas Gerais, Brazil

Abstract

This study explored the volatile organic compounds (VOCs) of Irpex laceratus, a novel endophytic fungus isolated from Glaucium fimbrilligerum Boiss. Metabolites were extracted with ethyl acetate, diluted in n-hexane and n-butanol solvents, and identified by Gas Chromatography-Mass Spectrometry (GC-MS) using a non-polar column and NIST23 library matching (≥70%). The n-hexane extraction identified 47 distinct compounds, with simple hydrocarbons (40.42%) and organo-oxygen compounds (29.78%) being the most abundant classes. Other detected groups included organonitrogen (10.63%), organoiodine (6.38%), organosilicon (4.25%), and minor contributions from organochlorine, organosulfur, organobromine, and organofluorine compounds (each 2.12%). Most metabolites exhibited molecular weights between 200–400 g/mol, representing 44.67% of the total. In contrast, the n-butanol extract revealed 36 distinct compounds, with a different chemical distribution: simple hydrocarbons (33.33%), organo-oxygen compounds (27.77%), organosilicon (16.16%), organonitrogen and organochlorine (each 11.11%), and small proportions of organoarsenic, organosulfur, and organofluorine compounds (each 2.77%). The dominant molecular weight range for n-butanol metabolites was 200–300 g/mol (44.44%), followed by 300–400 g/mol (30.55%). The present study offers a comprehensive chemical profiling of I. laceratus, serving as a foundational contribution toward the systematic characterization of its VOC diversity. The identification of a broad spectrum of structurally and functionally diverse organic compounds underscores the metabolic complexity of the species and highlights its potential for discovering novel bioactive compounds.

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Main Subjects

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Mycologia Iranica
Volume 12, Issue 1
June 2025
Pages 95-105

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  • Receive Date: 30 April 2025
  • Revise Date: 18 June 2025
  • Accept Date: 27 June 2025

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