Taxonomic update of Erysiphe sect. Erysiphe (Erysiphaceae, Helotiales) in Iran using DNA barcoding and phylogenetic analysis

Document Type : Original Article

Authors

1 Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

10.22043/MI.2024.364811.1276

Abstract

Taxonomy and phylogeny of Erysiphe sect. Erysiphe (Erysiphaceae, Helotiales) in Iran was revised. One hundred samples from the University of Guilan Mycological Fungarium (GUM) and the fungal reference collection of the Ministry of Jihad-e Agriculture (IRAN), as well as newly collected specimens during 2019–2021, were investigated using morphology and ITS-LSU rDNA sequence analysis. Based on our findings, Erysiphe sect. Erysiphe has 22 accepted and two unknown species in Iran viz.: E. aquilegiae, E. betae, E.  buhrii, E.  caulicola, E.  circaeae, E.  convolvuli, E.  cruchetiana, E. cruciferarum, E.  heraclei, E. howeana, E.  limonii, E. lycopsidis, E.  malvae, E.  mayorii,  E.  medicaginis, E.  paeoniae, E. pisi, E. polygoni, E. punicae, E. rumicicola, E.  sedi, E. urticae. E. sedi and E. paeoniae are new records for the funga of Iran. In addition, Mesostemma kotschyana (Caryophyllaceae) is reported as a new host for E. buhrii. The occurrence of potential cryptic species on Urtica spp. is discussed as well

Keywords

References

Bradshaw, M., Tobin, P.C. 2020. Sequencing herbarium specimens of a common detrimental plant disease (powdery mildew). Phytopathology. 110(7):1248-54.
Bradshaw, M., Braun, U., Götz, M., Jurick, Ι.Ι.W. 2022. Phylogeny and taxonomy of powdery mildew caused by Erysiphe species on Lupinus hosts. Mycologia. 114(1): 76-88.
Braun, U. 1987. A monograph of the Erysiphales (powdery mildews). Beihefte zur Nova Hedwigia. 89:1–700  
Braun, U., Cook, R. 2012. Taxonomic manual of the Erysiphales. Utrecht: CBS–KNAW Fungal Biodiversity Centre.
Braun, U., Kruse, J., Wolcan, S.M., Murace, M. 2010. Three new species of the genus Erysiphe (Ascomycota, Erysiphales) on legumes and some new combinations. Mycotaxon. 112(1): 173-187.
Crous, P.W., Wingfield, M.J., Chooi, Y.H., Gilchrist, C.L., Lacey, E., Pitt J.I., Roets, F., Swart, W.J., Cano-Lira, J.F., Valenzuela-Lopez, N., Hubka, V. 2020. Fungal Planet description sheets: 1042–1111. Persoonia. 44:301.
Cunnington, J.H., Takamatsu, S., Lawrie, A.C., Pascoe, I.G. 2003. Molecular identification of anamorphic powdery mildews (Erysiphales). Australasian Plant Pathology. 32:421-428.
Darsaraei, H., Khodaparast, S.A., Takamatsu, S., Abbasi, M., Asgari, B., Sajedi, S., Götz, M., Liu, S.Y., Feng, J., Bradshaw, M., Bulgakov, T. 2021a. Phylogeny and taxonomy of the Erysiphe adunca complex (Erysiphaceae, Helotiales) on poplars and willows. Mycological Progress. 20:517-37.
Darsaraei, H., Khodaparast, S.A., Mousanejad, S., Asgari, B., Aliabadi, F., Sajedi, S. 2021b. A taxonomic revision of Erysiphe sect. Uncinula (Erysiphaceae, Helotiales) in Iran. Mycologia Iranica. 8(2):51-65.
Darsaraei, H. 2022. Phylogeny and taxonomy of the genus Erysiphe (Ascomycota: Erysiphaceae) in Iran. PhD. dissertation. University of Guilan, Faculty of Agricultural Sciences.
Darsaraei, H., Afshan, N.U.S., Afzal, S., Zafar, I., Anwar, A., Götz, M., Khodaparast, S., Braun, U. 2023a. A re-assessment of Erysiphe polygoni: Phylogenetic analyses suggest an undescribed species. Sydowia. 75:233-242. https://doi.org/10.12905/0380.sydowia75-2023-0233
Darsaraei, H., Khodaparast, S.A., Mousanejad, S., Sepahvand, K., Asgari, B., Sajedi, S. 2023b. Taxonomic update with DNA barcoding and phylogenetic analysis of Erysiphe sect. Microsphaera (Erysiphaceae, Helotiales) in Iran. Mycologia Iranica. 10(2):11-32. https://doi.org/10.22043/MI.2023.361185.1248
Darsaraei, H., Khodaparast, S.A., Asgari, B., Götz, M., · Takamatsu, S., ·Braun, U. 2023c. Erysiphe spp. on Fabaceae from Iran: A new insight into some complex species. Mycological Progress. https://doi.org/10.1007/s11557-024-01945-5
Davari, M., Sharifi, K., Khodaparast, S.A., Bagheri-Kheirabadi, M. 2015. First report of powdery mildew caused by Pseudoidium neolycopersici on Lycopersicon esculentum based on morphological and molecular identification in Iran. Iranian Journal of Plant Pathology. 51(3): 385–390.
Divarangkoon, R., Meeboon, J., Monkhung ,S., To-anun, C., Takamatsu, S. 2011. Two new species of Erysiphe (Erysiphales, Ascomycota) from Thailand. Mycosphere. 2(3): 231-238.
Francis, S. 2002. Sugar‐beet powdery mildew (Erysiphe betae). Molecular plant pathology. 3(3): 119-124
Hsiao, H.Y., Ariyawansa, H.A., Hsu, C.C., Wang, C.J., Shen, Y.M. 2022. New records of powdery mildews from Taiwan: Erysiphe ipomoeae comb. nov., E. aff. betae on Buckwheat, and E. neolycopersici comb. nov. on Cardiospermum halicacabum. Diversity. 14(3):204. https://doi.org/10.3390/d14030204
Kashimoto, K., Matsuda, Y., Matsutani, K., Sameshima, T., Kakutani, K., Nonomura, T., Okada, K., Kusakari, S.I., Nakata, K., Takamatsu, S., Toyoda, H. 2003. Morphological and molecular characterization for a Japanese isolate of tomato powdery mildew Oidium neolycopersici and its host range. Journal of General Plant Pathology. 69:176-85.
Katoh, K., Misawa, K., Kuma, K.I., Miyata, T. 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic acids research. 30(14):3059-66.
Khodaparast, S.A., Hedjaroude, G.A., Ershad, D.J., Zad, J., Termeh, F. 2000. A study on identification of Erysiphaceae in Gilan Province, Iran (I). Rostaniha. 1:53-63.
Khodaparast, S.A., Hedjaroude, G.A., Takamatsu, S. 2003. Phylogenetic relationships between Iranian isolates of Microsphaera and Erysiphe s. lat. based on rDNA internal transcribed spacers sequences. Rostaniha, 4(2): 93-104.
Khodaparast, S.A., Darsaraei, H., Abbasi, M. 2021. The genus Arthrocladiella: a new report of powdery mildew fungi from Iran. Mycologia Iranica. 8(2):135-40.
Kiss, L., Cook, R.T.A., Saenz, G.S., Cunnington, J.H., Takamatsu, S., Pascoe, I., Bardin, M., Nicot, P., Sato, Y., Rossman, A.Y. 2001. Identification of two powdery mildew fungi, Oidium neolycopersici sp. nov. and O. lycopersici, infecting tomato in different parts of the world. Mycological Research. 105(6):684-697.
Kumar, S, .Stecher, G., Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular biology and evolution. 33(7):1870-4.
Liu, L., Bradshaw, M., Braun, U., Götz, M., Khodaparast, S.A., Liu, T.Z., Bulgakov, T.S., Darsaraei, H., Hofbauer, W.K., Li, Y., Liu, S.Y. 2022a. Phylogeny and taxonomy of Erysiphe berberidis (s. lat.) revisited. Mycoscience. 63(5):222-34.
Liu L., Hui, L.C., Yu, S.R., Li, Y, Liu, S.Y. 2022b. Erysiphe ruyongzhengiana sp. nov., a new powdery mildew species on Aristolochia debilis, belonging to the Erysiphe aquilegiae clade. Mycoscience. 63(4): 169-175.
Meeboon, J., Hidayat, I., Takamatsu, S. 2012. Erysiphe javanica sp. nov., a new tropical powdery mildew from Indonesia. Mycotaxon. 120(1), 189-194.
Meeboon, J., Takamatsu, S. 2014. Notes on powdery mildews (Erysiphales) in Japan: I. Erysiphe sect. Erysiphe. Mycoscience. 56(3):257-66.
Mori, Y., Sato Y., Takamatsu, S. 2000. Evolutionary analysis of the powdery mildew fungi using nucleotide sequences of the nuclear ribosomal DNA. Mycologia. 92: 74-93.
Pastirčáková, K., Jankovics, T., Komáromi, J., Pintye, A., Pastirčák, M. 2016. Genetic diversity and host range of powdery mildews on Papaveraceae. Mycological Progress. 15:1-8.
Rogerson, C.T. 1987. Host range and geographical distribution of powdery mildew fungi. By Koji Amano (Hirata). Brittonia. 4(39):459.
Shin, H.D., Meeboon, J., Takamatsu, S., Adhikari, M.K., Braun, U. 2019. Phylogeny and taxonomy of Pseudoidium pedaliacearum. Mycological Progress.18:237-46.
Silvestro, D., Michalak, I. 2012. RaxmlGUI: a graphical front-end for RAxML. Organisms Diversity & Evolution. 12:335-7.
Takamatsu, S., Ito, H., Shiroya, Y., Kiss, L., Heluta, V. 2015. First comprehensive phylogenetic analysis of the genus Erysiphe (Erysiphales, Erysiphaceae) I. The Microsphaera lineage. Mycologia. 107(3):475-89.
Takamatsu, S., Taguchi, Y., Shin, H.D., Paksiri, U., Limkaisang, S., Binh, N.T., Sato, Y. 2002. Two Erysiphe species associated with recent outbreak of soybean powdery mildew: results of molecular phylogenetic analysis based on nuclear rDNA sequences. Mycoscience. 43(4): 333-341.
Tanda, S. 2000. Two new species of Erysiphaceae from Japan. Mycoscience 41(2): 155-160.
Walsh, S.P., Metzger, D.A.,  Higuchi, R. 2013. Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material. Biotechniques. 54(3):134-9.
Zhang, Y.J., Zhang, S., Liu, X.Z., Wen, H.A., Wang, M. 2010. A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Letters in Applied Microbiology. 51(1):114-8.
Mycologia Iranica

Articles in Press, Accepted Manuscript
Available Online from 07 June 2024

Files

Share

How to cite

Statistics