Green synthesis of nano-sized calcite crystals by ureolytic Acremonium egyptiacum IRAN 5247C associated with grapevine decline

Document Type : Original Article

Authors

1 Department of Biological Science, Faculty of Science, University of Kurdistan, P.O. Box 416, Sanandaj, Kurdistan, Iran

2 Department of Biological Science, Faculty of Science, University of Kurdistan, Sanadaj; Research Center for Nanotechnology, University of Kurdistan, Sanandaj, Kurdistan, Iran

3 Department of Plant Protection, Agriculture Faculty, University of Kurdistan, P.O. Box 416, Sanandaj, Kurdistan, Iran.University of Kurdistan

4 Department of Physics, College of science, University of Halabja, Kurdistan region, Iraq

5 Department of Plant Protection, Agriculture Faculty, University of Kurdistan, P.O. Box 416, Sanandaj, Kurdistan, Iran

Abstract

Nano-calcite, or calcium carbonate nanoparticles, is valued for its stability and versatile applications, particularly in agriculture, where it enhances soil quality, regulates pH, improves nutrient delivery, and promotes sustainable crop yields. In this research, nano-calcite was synthesized using Acremonium egyptiacum IRAN 5247C, isolated from grapevine necrotic wood, under sedimentary conditions through fungal urease production. Key factors including urea and calcium concentrations, pH, incubation time, and temperature were optimized using a one-factor-at-a-time (OFAT) approach, resulting in a maximum yield of 595 mg of calcite per 10 ml of solution. Field emission scanning electron microscopy (FE-SEM) imaging revealed that the nanocrystals were predominantly spherical, averaging 73.5 ± 8.02 nm in size, with a distribution range of 25 to 125 nm. X-ray diffraction (XRD) and Fourier transform infrared-Raman (FTIR-Raman) spectroscopy confirmed a well-defined crystalline calcite structure characterized by carbonate ions. This study reports the first synthesis of nano-calcite using Acremonium egyptiacum, with precise control over particle shape and size, an ideal feature for applications in concrete reinforcement, bio-cementation, and agriculture.

Keywords

Main Subjects

References

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Mycologia Iranica
Volume 11, Issue 2
December 2024
Pages 19-31

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History

  • Receive Date: 10 November 2024
  • Revise Date: 18 December 2024
  • Accept Date: 17 December 2024
  • Publish Date: 01 December 2024

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