Blumeriella jaapii, a new fungal species on sour cherry in Guilan province, Iran

Document Type : Short Article

Author

Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

Sour cherry trees in Guilan Province are infected by at least two fungi that cause leaf spots and shot holes namely Wilsonomyces carpophilus and Pruniphilomyces circumscissus. In the late summer and early autumn of 2023, a leaf spot in the form of small purple-red to brown lesions about 1-3 mm in size was seen on the sour cherry trees in Sumaehsara County, Guilan Province, Iran. Mature conidiomata, acervuli, were formed frequently on the abaxial leaf surface at the center of each lesion, producing asexual conidia in white masses. Morphological and molecular studies showed that Blumeriella jaapii is associated with the sour cherry leaf spot disease. This is the first report on this fungus and the first attempt to sequence the ITS-nrDNA as the primary barcode of Blumeriella jaapii in Iran.

Keywords

Main Subjects


Andersen, K. L., Sebolt, A. M., Sundin, G. W., and Iezzoni, A. F. 2018. Assessment of the inheritance of resistance and tolerance in cherry (Prunus sp.) to Blumeriella jaapii, the causal agent of cherry leaf spot. Plant Pathology 67(3): 682–691.
Bakhshi, M., Zare, R., Braun, U., and Taheri, H. 2021. Polyphasic taxonomy of four passalora-like taxa occurring on fruit and forest trees. Mycological Progress 20(9): 1157–1173.
Ershad, D. 2009. Fungi of Iran, Iranian Research Institute of Plant Protection, Iran.
Farr, D.F., Rossman, A.Y. and Castlebury, L.A. 2021. United States National Fungus Collections Fungus-Host Dataset. Ag Data Commons. https://doi.org/ 10.15482/USDA.ADC/1524414.
Holb, I.J. 2009. Some biological features of cherry leaf spot (Blumeriella jaapii) with special reference to cultivar susceptibility. International Journal of Horticultural Science 15(1–2): 91–93.
Karsten, P.A. 1884. "Symbolae ad mycologiam Fennicam. XVI." Meddelanden af Societas pro Fauna et Flora Fennica 11: 148–161.
Khan, N.A., Bhat, M.A., Ahmad, K. and Qazi, N.A. 2014. First record of cherry leaf spot caused by Cylindrosporium padi (P. Karst.) from India. Applied Biological Research 16(1):122–123.
Khan, K.A., Nabi, S.U. and Khan, N.A. 2016. Identification of Cylindrosporium padi associated with leaf spot disease of cherry in Kashmir Valley, India. Journal of Phytopathology & Pest Management 3(3): 43–52.
McManus, P.S., Proffer, T.J., Berardi, R., Gruber, B.R., Nugent, J.E. et al. 2007. Integration of copper-based and reduced-risk fungicides for control of Blumeriella jaapii on sour cherry. Plant Disease 91(3): 294–300.
Peng, J., Rojas, J.A., Sang, H., Proffer, T.J., Outwater, et al. 2020. Draft genome sequence resource for Blumeriella jaapii, the cherry Leaf spot pathogen. Phytopathology 110(9): 1507–1510.
Rossman, A.Y. and Johnston, P. 2016. Proposals to conserve the names Pseudopeziza jaapii (Blumeriella jaapii) against Cylindrosporium padi; Pyrenopeziza medicaginis (Leptotrochila medicaginis) against Sporonema phacidioides; Peziza cinnamomea (Pezicula cinnamomea) against Naemaspora grisea; and Pyrenopeziza brassicae against Cylindrosporium concentricum (Ascomycota: Leotiomycetes). Taxon 65(1): 184–285.
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, et al. 2012. Fiji: an open-source platform for biological-image analysis. Nature methods (7): 676–682.
Schuster, M. 2004. Sour cherry breeding–Resistance to leaf spot diseases, Blumeriella jaapii. In Ecofruit-11th International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-Growing: Proceedings to the Conference from 3rd February to 5th February 2004 at Weinsberg/Germany (pp. 169–173).
Sutton, B.C. 1980. The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. CMI Press, UK.
Tamura, K., Stecher, G., and Kumar, S. 2021. MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution https://doi.org/10.1093/ molbev/ msab120.
Tezean, H. 2008. An outbreak of cherry leaf spot (Blumeriella jaapii) on sweet cherry in Canakkale Province of Turkey. Acta Horticulture 795: 965–968.
Valiushkaite, A. 2002. Investigation of biological peculiarities of Blumeriella jaapii. Plant Protection Science 38 (SI2-6thConfEFPP): 399–401.
Vu, D., Groenewald, M., De Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., et al. 2019. Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies In Mycology 92(1): 135–154.
White, T.J., Bruns, T., Lee, S.J. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications (M.A. Innis, D.H. Gelfand, J.J. Sninsky & T.J. White, eds): 315–322. Academic Press, USA.
Zhang, Y.J., Zhang, S., Liu, X.Z., Wen, H.A., and Wang, M. 2010. A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Letters In Applied Microbiology 51(1): 114–118.
Volume 11, Issue 1
June 2024
Pages 123-128
  • Receive Date: 18 June 2024
  • Revise Date: 23 June 2024
  • Accept Date: 28 June 2024
  • Publish Date: 01 June 2024