Morphological and molecular characterization of Podosphaera minor on Spiraea in Iran

Document Type : Short Article

Authors

Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

10.22043/MI.2023.361225.1249

Abstract

Powdery mildew is a common disease of Spiraea spp. worldwide; however, there are no reports of this destructive disease in Spiraea species from Iran.  In recent years, severe powdery mildew symptoms have been observed in Spiraea spp. throughout the Guilan Province. In this study, we collected infected plant specimens and used morphological and molecular approaches to identify the powdery mildew fungus involved in this disease. We did not observe the teleomorphic state of the fungus in this area, and it appears that the fungus occurred only in the anamorphic state. At least six species of Podosphaera occur worldwide, and there is a considerable overlap between the anamorphic characteristics of some species. Hence, the ITS sequence was used to identify common powdery mildew species occurring in Spiraea in the Guilan Province. The results showed that the ITS sequence is useful for Podosphaera species in Spiraea. Podosphaera minor Howe. was identified as the causal agent of powdery mildew disease in this plant. To our knowledge, this is the first report of Podosphaera minor as a fungus in Iran.

Keywords

References

Anonymous. 2023. eflora.org. Retrieved January 15,
2023, from shttp://www.efloras.org.
Bradshaw M, Tobin PC. 2020. Sequencing herbarium
specimens of a common detrimental plant disease
(powdery mildew). Phytopathology 110: 1248–
1254.
Braun U, Cook R. 2012. Taxonomic manual of the
Erysiphales. Utrecht: CBS-KNAW Fungal
Biodiversity Centre.
Cunnington JH, Takamatsu S, Lawrie AC, Pascoe IG.
2003. Molecular identification of anamorphic
powdery mildews (Erysiphales). Australasian Plant
Pathology 32: 421–428.
Darsaraei H, Khodaparast SA, Takamatsu S, Abbasi
M, Asgari B, Sajedi S, Gotz M, Liu Sh, Feng J,
Bradshaw M, Bulgakov T, Braun U. 2021.
Phylogeny and taxonomy of the Erysiphe adunca
complex (Erysiphaceae, Helotiales) on poplars and
willows. Mycological Progress 20: 517–537.
Khodaparast SA, Darsaraei H, Abbasi M. 2021. The
genus Arthrocladiella: a new report of powdery
mildew fungi from Iran. Mycologia Iranica 8: 135–
140.
Farr DF, Rossman AY. 2023. Fungal Databases, U.S.
National Fungus Collections, ARS, USDA.
Retrieved January 15, 2023, from https://nt.arsgrin.gov/fungaldatabases.
Kumar S, Stecher G, Tamura K. 2016. MEGA7:
Molecular Evolutionary Genetics Analysis Version
7.0 for Bigger Datasets. Molecular Biology and
Evolution 33: 1870–1874.
Silvestro D, Michalak I. 2012. raxmlGUI: a graphical
front-end for RAxML. Organisms Diversity &
Evolution 12:335–337.
Takamatsu S, Niinomi S, Harada M, Havrylenko M.
2010. Molecular phylogenetic analyses reveal a
close evolutionary relationship between
Podosphaera (Erysiphales: Erysiphaceae) and its
rosaceous hosts. Persoonia-Molecular Phylogeny
and Evolution of Fungi 24: 38–48.
Zhang YJ, Zhang S, Liu X, Wen HA, Wang M. 2010.
A simple method of genomic DNA extraction
suitable for analysis of bulk fungal strains. Letters
in Applied Microbiology 51: 114–118
Mycologia Iranica
Volume 10, Issue 1
June 2023
Pages 103-108

Files

Share

How to cite

Statistics