Variation among Fusarium oxysporum f. sp. ciceris isolates causing chickpea root and crown rot from Kurdistan province

Document Type : Original Article

Authors

1 Department of Plant Protection, Faculty of Agronomic Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

2 Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

3 Agricultural and Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Shahrekord, Iran.

10.22043/MI.2023.359141.1239

Abstract

Fusarium wilt, root and crown rot caused by 
Fusarium oxysporum f. sp. ciceris, (FOC) is the highly 
significant soil-borne disease of chickpea in the 
Kurdistan province of Iran. The distribution of 
pathogenic races of FOC in Kurdistan province was 
determined during this research. Infected plant 
samples were collected from 42 fields in the chickpea 
production area of the Kurdistan province. The 
causative microorganism of the disease was isolated 
and purified from each sample, and then FOC isolates 
were identified by morphological characters. After the 
pathogenicity test and evaluation of pathogenic 
variability on the susceptible cultivar Kaka, the DNA 
extraction, the molecular identification of species, and 
races of pathogenic isolates were performed using 
FOC-specific linked primers. Among the collected 
isolates, 37 were identified as Fusarium oxysporum f. 
sp. ciceris. Molecular identification of races using 
SCAR-linked markers (1B/C, 0, 2,3,4,5, 6, and 1A) 
revealed that 28 out of 37 isolates belonged to race 0, 
and other isolates belonged to race 1B/C. There was no 
relationship between the prevalence of races and their 
geographical distribution. Identification of the races is 
crucial for the evaluation of resistance and the 
development of new commercial cultivars. The 
application of resistant cultivars is a fundamental 
approach for the integrated management of the 
Fusarium wilt, root, and crown rot for durable chickpea 
production

Keywords

References

Alloosh M, Hamwieh A, Ahmed S, Alkaei B. 2019. Genetic diversity of Fusarium oxysporum f. sp. ciceris isolates affecting chickpea in Syria. Crop Protection, 124: 104-863.
Arvayo-Ortiz RM, Esqueda M, Aedo-Flix M. 2011. Morphological variability and races of Fusarium oxysporum f. sp. ciceris associated with chickpea (Cicer arietinum) crops. American Journal of Agricultural and Biological Sciences, 6: 114-121.
Bayraktar H, Dolar FS, Maden S. 2008. Use of RAPD and ISSR markers in detection of genetic variation and population structure among Fusarium oxysporum f. sp. ciceris isolates on chickpea in Turkey. Journal of Phytopathology, 156: 146-154.
Bayraktar H, Dolar FS. 2012. Pathogenic variability of Fusarium oxysporum f. sp. ciceris isolates from chickpea in Turkey. Pakistan Journal of Botany, 44: 821-823.
Dandale S, Mane SS, Ingle ST, Jadhav PV, Patil AN, Nandanwal RS, Jankar KB, Tatte RR, Kalane PN. 2021. Pathogenic variability among specific isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. The Pharma Innovation Journal, 10: 20-28.
Dubey SC, Singh SR, Singh B. 2010. Morphological and pathogenic variability of Indian isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. Archives of Phytopathology and Plant Protection, 43: 174-190.
Gurjar G, Barve M, Giri A, Gupta V. 2009. Identification of Indian pathogenic races of Fusarium oxysporum f. sp. ciceris with gene specific, ITS and random markers. Mycologia, 101: 484-495.
Halila M, Strange R. 1996. Identification of the causal agent of wilt of chickpea in Tunisia as Fusarium oxysporum f. sp. ciceri race 0. Phytopathologia mediterranea, 35: 67-74.
Haware M, Nene Y. 1982. Races of Fusarium oxysporum f. sp. ciceri. Plant Disease, 66(9): 809-810.
Jalali B, Chand H. 1992. Chickpea wilt. Plant diseases of international importance, 1: 429-444.
Jiménez-Díaz, RM, Trapero-Casas, A, de la Colina, JC.1989. Races of Fusarium oxysporum f.sp. ciceri Infecting chickpeas in southern Spain. In: Tjamos, E.C., Beckman, C.H. (eds) Vascular Wilt Diseases of Plants. NATO ASI Series, Vol 28. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73166-2_39.
Jiménez-Díaz RM, Alcala-Jimenez A, Hervas A, Trapero-Casas J-L. 1993. Pathogenic variability and host resistance in the Fusarium oxysporum f. sp. ciceris-Cicer arietinum pathosystem. Hodowla Roślin, Aklimatyzacja i Nasiennictwo, 37: 87-94.
Jimenez-Gasco MDM, Pérez-Artés EM. 2001. Identification of pathogenic races 0, 1B/C, 5, and 6 of Fusarium oxysporum f. sp. ciceris with random amplified polymorphic DNA (RAPD). European Journal of Plant Pathology, 107: 237-248.
Leslie J, Summerell B. 2006. The Fusarium laboratory Manual. Blackwell Publishing Professional, USA, 388pp.
Mohamed OE, Hamwieh A, Ahmed S. 2015. Genetic Variability of Fusarium oxysporum f. sp. ciceris Population Affecting Chickpea in the Sudan. Journal of Phytopathology, 163: 941-946.
Montakhabi MK, Shahidi Bonjar GH, Talebi R. 2018. Genetic diversity and population structure of Iranian isolates of Fusarium oxysporum f. sp. ciceris, the causal agent of chickpea wilt, using ISSR and DAMD-PCR markers. Journal of Experimental Biology, 16: 291-298.
Montakhabi MK, Shahidi Bonjar GH, Talebi R. 2020. Mating type profiling and SSR-based genetic diversity analysis of Iranian Fusarium oxysporum f. sp. ciceris causing wilt in chickpea. International Journal of Agriculture and Biology, 23: 1101-1106.
Nelson PE, Toussoun TA, Marasas WFO. 1983. Fusarium species: an illustrated manual for identification. The Pennsylvania State University. USA, 198pp.
Nene Y, and Haware, M. 1980. Screening chickpea for resistance to Wilt. Plant Disease 64: 379-380.
Nene YL. 1988. Multiple-disease resistance in grain legumes. Annual Review of Phytopathology‚ 26: 203-217.
Sharma M, Nagavardhini A, Thudi M, Ghosh R, Pandeh S, Varshney RK. 2014. Development of DArT markers and assessment of diversity in Fusarium oxysporum f. sp. ciceris, wilt pathogen of chickpea (Cicer arietinum L.). BMC genomics, 15: 1-14.
Shehabu M, Ahmed S, Sakhuja PK. 2008. Pathogenic variability in Ethiopian isolates of Fusarium oxysporum f. sp. ciceris and reaction of chickpea improved varieties to the isolates. International journal of pest management, 54: 143-149.
Shokri J, Javan-Nikkhah M, Rezaee S, Zamanizadeh HR, Nourollahi KH. 2020. Molecular identification of the races of Fusarium oxysporum f. sp. ciceris, causal agent of chickpea wilt in western and north western provinces of Iran. Applied Entomology and Phytopathology, 88: 11-22.
Tekeoglu M, Özkilinc H,  Tunali B. 2017. Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey. Mediterranean Agricultural Sciences, 30: 27-33.
Trapero-Casas A, Jiménez Díaz R M. 1985. Fungal wilt and root rot diseases of chickpea in southern Spain. Phytopathology, 75:1146-1151.
Zamani K, Faghihi K,  Tofighi T. 2004. Synthesis and antimicrobial activity of some pyridyl and naphthyl substituted 1, 2, 4-triazole and 1, 3, 4-thiadiazole derivatives. Turkish Journal of Chemistry, 28: 95-100.
Zhong S, Steffenson BJ. 2001. Virulence and molecular diversity in Cochliobolus sativus. Phytopathology, 91: 469-476.
Mycologia Iranica
Volume 9, Issue 1
June 2022
Pages 85-95

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