Morphological and molecular characterization of a novel Pestalotiopsis trachycarpicola, causing Garden Croton leaf spot in Iran

Document Type : Original Article

Authors

1 Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran

2 Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

10.22043/MI.2022.360270.1236

Abstract

In the spring of 2021, leaf spot disease symptoms were observed in the Garden Croton (Codiaeum variegatum) plants in one of the ornamental plant greenhouses of Mahallat County, Markazi Province, Iran. To identify the causal agent of the disease, infected plant leaves sampled and then were transferred to the mycology laboratory and nine fungal isolates were recovered. The fungal isolates were characterized based on the morphological features and molecular data using the combined sequences of the internal transcribed spacer (ITS) regions and the translation elongation factor 1-alpha (tef) gene for a representative isolate UT2022. According to the morphology and phylogenetic analysis, the isolate UT2022 was identified as Pestalotiopsis trachycarpicola. The pathogenicity of the isolate UT2022 was performed on healthy and growing leaves of the C. variegatum plants. Inoculated leaves showed leaf spot symptoms 14 days after the inoculation, while the leaves of the control plants were symptomless. To complete Koch’s postulate, P. trachycarpicola was re-isolated from the newly produced leaf spots. Based on the bibliography, P. trachycarpicola is reported as a new taxon for fungi of Iran, as well as P. trachycarpicola is reported as the causal agent of the leaf spot symptom of the C. variegatum in the world.

Keywords

References

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. Journal of Molecular Biology 215: 403-410.
Carbone I, Kohn LM. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553–556.
Das K, Lee SY, Jung HY. 2021. Pestalotiopsis kaki sp. nov., a novel species isolated from Persimmon Tree (Diospyros kaki) Bark in Korea. Mycobiology 491: 54-60.‏
Deng M, Chen J, Henny RJ, Li Q. 2010. Genetic relationships of Codiaeum variegatum cultivars analyzed by amplified fragment length polymorphism markers. HortScience 45: 868-874.‏
Dutta AC 2004. Botany for Degree Students, 6th ed, Oxford University Press, pp. 415-417, 592.
Ershad D. 2022. Fungi and Fungal Analogues of Iran. Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization, Tehran, Iran, 712 pp.
Fan J, Qiu HB, Long HJ, Zhao W, Xiang XL, Hu AL. 2021. First report of leaf spot on sorghum caused by Pestalotiopsis trachycarpicola in China. Journal of Plant Pathology 103: 1043-1044.‏
Farr DF, Rossman, AY. 2022. Fungal Databases, U.S. National Fungus Collections, ARS, USDA. Available at: https://nt.ars-grin.gov/fungaldatabases.
Felsenstein J. 1973. Maximum likelihood and minimum-steps methods for estimating evolutionary trees from data on discrete characters. Systematic Biology 22: 240-249.
Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35: 1547-1549.
Li L, Yang Q, Li H. 2021. Morphology, Phylogeny, and Pathogenicity of Pestalotioid Species on Camellia oleifera in China. Journal of Fungi 7: 1080.‏
Liu XM, Wei YX, Zhang H, Zhou FX, Pu JJ. 2015. First report of powdery mildew (Pseudoidium neolycopersici) on Croton (Codiaeum variegatum var. pictum) in China. Plant Disease 99: 288-288.‏
Maharachchikumbura SSN, Hyde KD, Groenewald JZ, Xu J, Crous PW. 2014. Pestalotiopsis revisited. Stud Mycol. 79:121–186.
Magdalita PM, Torreta NK, Sotto RC. 2014. Characterization of phenotypic variation in selected Croton [Codiaeum variegatum (L.) Rhumph. ex A. Juss.] varieties and natural mutants. Journal of Nature Studies 13: 41-55.‏
Mollick AS, Shimoji H, Denda T, Yokota M, Yamasaki H. 2011. Croton Codiaeum variegatum (L.) Blume cultivars characterized by leaf phenotypic parameters. Scientia Horticulturae 132: 71-79.‏
Mwine JT, Van Damme P. 2011. Why do Euphorbiaceae tick as medicinal plants? A review of Euphorbiaceae family and its medicinal features. Journal of medicinal plants research 5: 652-662.‏
Nasib A, Ali K, Khan S. 2008. In vitro propagation of croton (Codiaeum variegatum). Pakistan Journal of Botany 40, 99-104.‏
Refaei J, Jones EBG, Sakayaroj J, Santhanam J. 2011. Endophytic fungi from Rafflesia cantleyi: species diversity and antimicrobial activity. Mycosphere 2: 429–447.
Silva AC, Diogo E, Henriques J, Ramos AP, Sandoval-Denis M, Crous PW, Bragança H. 2020. Pestalotiopsis pini sp. nov., an Emerging Pathogen on Stone Pine (Pinus pinea L.). Forests 11: 805.‏
Tempesta S, Rubini A, Franco P, Rambelli A. 2011. Pestalotiopsis endophytes from leaves of two orchid species collected in Costa Rica. Cryptogamie Mycologie, 32: 315-321.‏
Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673-4680.
White TJ, Bruns T, Lee SJWT, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18: 315-322.‏
Wikee S, Lombard L, Crous PW, Nakashima C, Motohashi K, Chukeatirote E, Alias SA, McKenzie EH, Hyde KD. 2013. Phyllosticta capitalensis, a widespread endophyte of plants. Fungal Diversity 60: 91–105.
Zhang Y, Maharachchikumbura SS, Mckenzie EH, Hyde KD. 2012. A novel species of Pestalotiopsis causing leaf spots of Trachycarpus fortunei. Cryptogamie Mycologie 33: 311-318.‏
Zhang X, YangY, Li S, Yan K, Li X. 2021. First report of leaf spots on Gentiana rhodantha caused by Pestalotiopsis trachycarpicola. Journal of General Plant Pathology 87: 316-321.‏
Zhong S, Steffenson BJ. 2001. Virulence and molecular diversity in Cochliobolus sativus. Phytopathology 91: 469-476.
Mycologia Iranica
Volume 9, Issue 1
June 2022
Pages 59-66

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