New species of family Saprolegniaceae in Mazandaran, Iran

Document Type : Original Article


Department of Plant Protection, Faculty of Agronomic Sciences, Sari Agricultural Sciences and Natural Resources University,Sari , Iran


Oomycete species occupy many different 
environments and many ecological niches. The 
family Saprolegniaceae from Oomycota includes 
widely distributed water molds which usually behave 
as saprophytes on plants and animal debris. Members 
of some species may also be pathogenic for plants and 
fish. Oomycete species identification based on DNA is 
well established, but DNA barcoding with cytochrome 
c oxidase subunit I (COXI) and II (COX II) are a 
relatively new approach. In this study, 57 isolates were 
obtained from water samples in mazandaran province, 
Iran. After morphological identification by 
morphological keys, ITS, COXI , and COX II gene 
regions of 10 representatives of the isolates were 
sequenced and 3 genera and 6 species (Newbya 
recurva, Achlya bisexualis, Dictyuchus monosporus, 
Saprolegnia ferax, S. bulbosa , and S. debaryana) 
were identified through BLASTn in NCBI gene bank. 
The results described in this paper were indicated 
that except for ITS, COXI and COXII sequencing 
could also be valuable resources 
to Saprolegniaceae identification. Except for S. ferax, 
other described species were new reports for oomycete 
biota of Iran.