Morphological and molecular characterization of Uzbekistanica spp. isolated from walnut trees

Document Type : Original Article


Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran


During surveys from walnut orchards of Hamedan and Kermanshah provinces, black spots on twigs of walnut trees were observed between 2018 and 2019. Due to the prevalence of these symptoms on the twigs of walnut trees, the samples were transported to the mycology laboratory for further investigations. After isolation and purification on PDA medium, 19 isolates were obtained from symptomatic twigs. Microscopic observations were conducted for grouping and morphological identifications. Based on morphological traits, two representative isolates were selected for molecular identifications. Molecular identification based on Internal Transcribed Spacer (ITS) region, indicated that these isolates were Uzbekistanica vitis-viniferae and U. yakutkhanika. Pathogenicity examinations were showed these isolates were pathogenic on walnut shoots in the laboratory conditions. To the best of our knowledge, this is the first report of Uzbekistanica as a new genus for Funga of Iran. Moreover, walnut tree is new host for U. vitis-viniferae and U. yakutkhanika.


Bagherabadi S, Zafari D, Soleimani, MJ. 2017. Morphological and molecular identification of Cytospora chrysosperma causing canker disease on Prunus persica. Australasian Plant Disease Notes 12: 26.‏
Cannon PF, Kirk, PM. 2007. Fungal families of the world. 1st edn. CABI, UK.
Crous PW, Wingfield MJ, Schumacher RK, Akulov A, Bulgakov, TS, Carnegie AJ, ... & Groenewald J Z. 2020. New and interesting fungi. 3. Fungal Systematics and Evolution 6: 157.‏
Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.
Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In Nucleic acids symposium series 41: 95-98. [London]: Information Retrieval Ltd., c1979-c2000.
Ho WC, Ko WH. 1997. A simple method for obtaining single-spore isolates of fungi. Botanical Bulletin of Academia Sinica 38.‏
Hyde KD, Dong Y, Phookamsak R, Jeewon R, Bhat DJ, Jones EB, ... & Sheng J. 2020. Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal diversity 100: 5-277.‏
Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111-120.
Kumar S, Stecher G, Tamura K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870-1874.
Möller EM, Bahnweg G, Sandermann H, Geiger HH. 1992. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic acids research 20: 6115.‏
Shah RA, Bakshi P, Sharma N, Jasrotia A, Itoo H, Gupta R, Singh A. 2021. Diversity assessment and selection of superior Persian walnut (Juglans regia L.) trees of seedling origin from North-Western Himalayan region. Resources, Environment and Sustainability 3: 100015.‏
Wanasinghe DN, Phukhamsakda C, Hyde KD, Jeewon R, Lee HB, Jones EG., ... & Karunarathna SC. 2018. Fungal diversity notes 709–839: taxonomic and phylogenetic contributions to fungal taxa with an emphasis on fungi on Rosaceae. Fungal diversity 89: 1-236.‏