Morphological and molecular characterization of Arbuscular Mycorrhizal fungus, Acaulospora punctata in Iran

Document Type : Original Article

Authors

Department of Plant Protection, Faculty of Agriculture, Vali-E-Asr University, Rafsanjan, Iran

Abstract

A new species of Acaulospora was found in the mountainous region of Iran (Kerman province, Raviz), at 2118 m asl around the roots of Prunus cerasus. The new species was named A. punctata by Ohel, 2013, because of its ornamentation on the outer spore wall with point-like. The spores are yellow-white, globose to subglobose, 105–139 µm in diameter, and have three walls that each of which consists of some layers. Phylogenetic analyses of sequences obtained from the ITS and partial LSU & SSU of the ribosomal genes confirm the new species in a separate clade within the Acaulosporaceae. According to the phylogenetic analysis, the fungus as named b-pooneh placed in a monophyletic group within the genus Acaulospora next to Acaulospora covernata, and A. ignota.

Keywords

References

Abeyrathne P, Nazar R. 2005. Parallels in rRNA processing: conserved features in the processing of the internal transcribed spacer 1 in the pre-rRNA from Schizosaccharomyces pombe. Biochemistry 44: 16977-16987
Aminian P, Sedaghati E, Hosseini S, Saberi R. 2021. Identification of arbuscular mycorrhizal fungi associated with different plants in Rafsanjan based on morphological characteristics and ß-tubulin Gene sequence. Biological Journal of Microorganisms 10: 27-40.
Aminizadeh S, Allaee H, Sedaghati E, Moradi M. 2018. Arbuscular mycorhizal fungi associated with pistachio trees in Rafsanjan. Journal of Pistachio Science and Technology 1: 1-21.
Błaszkowski J, Chwat G, Góralska A. 2015. Acaulospora ignota and Claroideoglomus hanlinii, two new species of arbuscular mycorrhizal fungi (Glomeromycota) from Brazil and Cuba. Mycological Progress 14: 1-11.
Błaszkowski J. 1995. Acaulospora koskei, a new species in Glomales from Poland. Mycological Research 99: 237-240.
Brundrett, M. 1994. Clearing and staining mycorrhizal roots. Practical Methods in Mycorrhiza Research: 42-46.
Calvet C, Estaún V, Camprub A, Hernández-Dorrego A, Pinochet J, Moreno M. 2004. Aptitude for mycorrhizal root colonization in Prunus rootstocks. Scientia Horticulturae 100: 39-49.
Crooks G. E, Hon G, Chandonia M, Brenner S. E. 2004. WebLogo: a sequence logo generator. Genome Research 14: 1188-1190.
Furrazola E, Goto B, Silva G, Torres-Arias Y, Morais T, C. Lima. 2013. Acaulospora herrerae, a new pitted species in the Glomeromycetes from Cuba and Brazil. Nova Hedwigia 97: 401-413.
Goto B, Silva G, Assis D, Silva D, Souza R, Ferreira A. 2012. Intraornatosporaceae (Gigasporales), a new family with two new genera and two new species. Mycotaxon 119: 117-132.
Hall I. 1984. Taxononmy of VA mycorrhizal fungi. In VA Mycorrhizae, CRC Press, Boca Raton, Florida.
Hempel S, Renker C, Buscot F. 2007. Differences in the species composition of arbuscular mycorrhizal fungi in spore, root and soil communities in a grassland ecosystem. Environmental Microbiology 9: 1930-1938.
Huang X, Madan A. 1999. CAP3: A DNA sequence assembly program. Genome Research 9: 868-877.
Krüger M, Stockinger H, Krüger C, Schüßler A. 2009. DNA‐based species level detection of Glomeromycota: one PCR primer set for all arbuscular mycorrhizal fungi. New phytologist 183: 212-223.
Maddison W. 2005. Mesquite: a modular system for evolutionary analysis. Version 1.06. http://mesquiteproject. org.
Morton J. 1986. Three new species of Acaulospora (Endogonaceae) from high aluminum, low pH soils in West Virginia. Mycologia 78:641-648.
Nguyen L, Schmidt A, Von Haeseler A, Minh B. 2015. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32: 268-274.
Oehl F, Sieverding E, Palenzuela J, Ineichen K, Silva G. 2011a. Advances in Glomeromycota taxonomy and classification. IMA fungus 2: 191-199.
Oehl F, Silva G, Palenzuela J, Sánchez-Castro I, Castillo C, Sieverding E. 2011b. Acaulospora punctata, a new fungal species in the Glomeromycetes from mountainous altitudes of the Swiss Alps and Chilean Andes. Nova Hedwigia 93: 353-362.
Oehl F, Sykorova Z, Redecker D, Wiemken A, Sieverding E. 2006. Acaulospora alpina, a new arbuscular mycorrhizal fungal species characteristic for high mountainous and alpine regions of the Swiss Alps. Mycologia 98: 286-294.
Oehl, F, Silva G, Palenzuela J, Sánchez-Castro I, Castillo C, Sieverding E. 2011. Acaulospora punctata, a new fungal species in the Glomeromycetes from mountainous altitudes of the Swiss Alps and Chilean Andes. Nova Hedwigia 93: 353-362.
Ohsowski B, Zaitsoff MP, Öpik M, Hart M. 2014. Where the wild things are: looking for uncultured Glomeromycota. New Phytologist 204: 171-179.
Palenzuela J, Azcón-Aguilar C, Barea J, Silva G, Oehl F. 2013. Acaulospora pustulata and Acaulospora tortuosa, two new species in the Glomeromycota from Sierra Nevada National Park (southern Spain). Nova Hedwigia 97: 305-319.
Palenzuela J, Azcón-Aguilar C, Barea J, Silva G, Oehl F. 2014. Acaulospora viridis, a new species in the Glomeromycetes from two mountain ranges in Andalucía (Spain). Nova Hedwigia 99: 71-82.
Rangwala S, Kuznetsov A, Ananiev V, Asztalos A, Borodin E, Evgeniev V. 2021. Accessing NCBI data using the NCBI sequence viewer and genome data viewer (GDV). Genome Research 31: 159-169.
Ronquist F, Teslenko M, Van Der Mark P, Ayres D, Darling A, Höhna S. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539-542.
Senés-Guerrero C & Schüßler A. 2016. DNA-based characterization and identification of arbuscular mycorrhizal fungi species. Microbial Environmental Genomics (MEG) Springer: 101-123.
Shen W, Le S, Li Y, Hu F. 2016. SeqKit: a cross-platform and ultrafast toolkit for FASTA/Q file manipulation. PloS one 11: e0163962.
Sieverding E, Friedrichsen J, Suden W. 1991. Vesicular-arbuscular mycorrhiza management in tropical agrosystems. Sonderpublikation der GTZ (Germany).
Stürmer S & Morton J. 1999. Taxonomic reinterpretation of morphological characters in Acaulosporaceae based on developmental patterns. Mycologia 91: 849-857.
Summuna B & Morton J. 2019. Diversity and distribution of arbuscular mycorrhizal fungi in pome and stone fruit orchards of North Western Himalayan State of Jammu & Kashmir. Indian Phytopathology 72: 343-350.
Schoch C, Seifert K, Huhndorf S, Robert V, Spouge J, Levesque C. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences 109: 6241-6246.
Trappe J. 1977.  Three new endogonaceae: Glomus constrictus, Sclerocystis clavispora, and Acaulospora scrobiculata. Mycotaxon 6: 359-366.
Yazdanpanah M, Sedaghati E, Khodygan P, Alaei H, Nadi M. 2017. Molecular and morphological identification of arbuscular mycorrhizal fungi associated with pistachio roots in Kerman province. Journal of Pistachio Science and Technology 2: 11-29.
Zangeneh S. 2021. Some new species of the genus Acaulospora from Iran. Rostaniha 22:67-74.
Mycologia Iranica
Volume 8, Issue 1
June 2021
Pages 69-77

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