Molecular identification of some anamorphic powdery mildews (Erysiphales) in Guilan province, north of Iran

Document Type : Original Article


Department of Plant Protection, College of Agricultural Sciences, University of Guilan, Rasht


In this study, ITS–rDNA region was used to identify some anamorphic powdery mildews in Guilan province. According to the results, Erysiphe species on Vicia faba and Sesbania punicea showed 100% similarity to each other, however, without ITS sequence of holotype of E. sesbaniae it is impossible to make conclusion whether Vicia faba powdery mildew fungus actually belongs to E. sesbaniae or E. trifoliorum complex. ITS sequence from isolate of Lagerstroemia indica powdery mildew showed 100% similarity to E. australiana. Podosphaera on Vigna fells into phylogenetic group containing P. xanthii on cucurbitaceous hosts. ITS sequence of chamomile (Matricaria chamomilla) powdery mildew fungus showed 100% similarity to P. xanthii on Xanthium strumarium. Molecular characteristics and morphological examination of conidia and conidia germination clearly showed that Dahlia powdery mildew in Guilan province is conspecific with G. spadiceus. Anamorph morphology and ITS sequence of Podosphaera on Epilobium and Erysiphe on Platanus orientalis showed that these species belong to P. epilobii and Erysiphe platani respectively.


Main Subjects


Powdery mildews are a group of obligate fungi that are well–characterized by their appearance on plant surface as white powdery spots on the leaves, stems, flowers and fruits. Powdery growth may cover whole plant leaves, stems, so that sometimes bushes become completely white. In the early growth stage, fungal structures on plant surface include mycelia, conidiophores and large numbers of asexual spores (conidia). As the disease progresses, sexual structures (chasmothecia) begin to appear as small black structures on the mycelium tomentum. All structures produced in both asexual and sexual stages along with host range information are usually critical and are often required for exact taxonomic treatment and identification of powdery mildew species (Braun 1987, Braun & Cook 2012, Shin 2009). However, there are many species that produce only asexual or sometimes sexual morphs. There are circumstances in which sexual stage in powdery mildew being absent. Researchers have shown that some species are heterothallic and two mating types are necessary for chasmothecia production (White 1970, Coyier 1973). In Australia the sexual stage of powdery mildew fungi has been recorded for only 20 out of 100 species known which attributed to the lack of appropriate mating strain (Cunnington et al. 2003). Identification being more complicated when sexual stages are unavailable to researchers, because currently nearly all identification keys have basically been provided according to sexual morph (Braun & Cook 2012). Although some mycologists have tried to develop identification keys for asexual powdery mildew fungi (Boesewinkel 1980, Cook et al.1997) but such taxonomic experience was not generally accepted and no longer being useful for species identification purposes. Moreover, some characters of powdery mildews are not accessible when dried herbarium materials are used such as conidium germination, exact measurement and description of conidia, conidiophores and appressoria. Hence, identification of some species remains usually difficult or problematic based solely on asexual stage morphology.

Host range and distribution of powdery mildew fungi in Iran has previously been treated by Khodaparast & Abbasi (2009). According to this paper, a total of 90 species of the Erysiphales have been identified in Iran, for some species no sexual stage is known. In Guilan province about 50 species occurs on several plant species, however, some of them have only been identified based on asexual stage (Khodaparast 2007).

The ribosomal DNA internal transcribed spacer (ITS) regions are useful for identifying powdery mildew fungi at species level (Hirata & Takamatsu 1996, Hirata et al. 2000, Ito & Takamatsu 2010, Khodaparast et al. 2001, 2007, 2012, 2016). It could represent a useful region for linking anamorphic specimens with their respective teleomorphs. In this study we used ITS region to identify some anamorphic powdery mildews in Guilan province, Iran.



Morphological examination

To observe the hyphae, conidiophores and conidia, clear adhesive tapes was used to strip off these structures from the leaf surface. A solution consisting of equal amounts of glycerol and lactic acid was used for mounting the fungal structures (Heidari et al. 2015). An Olympus light microscope (BH–2, Japan) equipped with a Sony digital camera was used for microscopic observations. All measurements were based on at least 20 to 30 observations. Morphological features of asexual states of the species were compared to the description of related taxa available in Braun & Cook (2012).

DNA sequencing and data analysis

Total DNA was isolated from fungal specimens by the Chelex method that had previously been used by several researchers (Walsh et al. 1991; Hirata and Takamatsu 1996; Khodaparast et al. 2001, 2007, 2012, 2016a, 2016b). Universal primers ITS1 (5'–TCCGTA GGTGAACCTGCGG–3') and ITS4 (5'–TCCTCCGC TTATTGATATGC–3') were used for amplification and sequencing of the fungal internal transcribed spacers (White et al. 1990).

The nucleotide sequences of the polymerase chain reaction (PCR) products were obtained using direct sequencing in an ABI 3730xl sequencer (Applied Biosystems, USA). Sequences were analyzed and edited using MEGA 7.0 (Kumar et al. 2016). Sequences were compared with the sequences available in the NCBI GenBank nucleotide database using a BLASTN search method. Several sequences from GenBank were selected for phylogenetic analyses. Sequences alignment was performed using muscle plug–in of MEGA 7.0 with the default settings (Edgar 2004). Phylogenetic trees were obtained using the minimum–evolution (Rzhetsky & Nei 1992) method in MEGA 7.0 (Kumar et al. 2016). In the ME method, the evolutionary distances were computed using the Kimura 2–parameter method (Kimura 1980). The ME tree was searched using the close–neighbour–interchange (CNI) algorithm at a search level of 1 (Nei & Kumar 2000). All ambiguous positions were removed for each sequence pair. All nucleotide substitutions were equally weighted and unordered. The neighbor–joining algorithm was used to generate the initial tree (Saitou & Nei 1987). The strength of the internal branches from the resulting trees was statistically tested by bootstrap analysis with 1000 replicates (Felsenstein 1985). All ITS sequences generated in this study are deposited in GenBank under accession numbers MF663773–MF663781.


Powdery mildew fungi collected in this study belong to different genera including Erysiphe section Erysiphe, E. sect. Microsphaera, E. sect. Uncinula, Podosphaera and Golovinomyces. ITS sequences were used in phylogenetic analysis. Due to the presence of two different phylogenetic groups of species belonging to euoidium and pseudoidium types, two different data matrices were used for analysis and resulting trees were shown in Fig. 1–2. Further results are presented here for each species.


Erysiphe on Sesbania and Vicia

According to Braun et al. (2010) and Braun & Cook (2012) three powdery mildew species including E. sesbaniae Wolcan & U. Braun 2010, Pseudoidium fabacearum (Hosag.) U. Braun & R.T.A. Cook, Microidium agatidis (É.E. Foëx) U. Braun 2012 have been recorded on Sesbania spp. M. agatidis is well–characterized by having small and catenate conidia. Two remaining species are morphologically very similar. However, based on description available in Braun & Cook (2012) E. sesbaniae differs in having two types of conidia and amphigenous mycelium. Mycelium of the examined specimens was amphigenous, usually covering most part of leaves. Conidia were more or less of two types. Such characteristics resemble those of E. sesbaniae. Another fungus was collected on heavily infected leaves of Vicia faba in greenhouse which possess anamorph characteristics similar to Sesbania. Moreover, ITS sequence of this taxon showed 100% similarity to the Sesbania powdery mildew fungus. Four Erysiphe species have been recorded on Vicia spp. viz. E. baeumleri (Magnus) U. Braun & S. Takam., E. ludens (E.S. Salmon) U. Braun & S. Takam., E. pisi DC. and E. viciae–unijugae (Homma) U. Braun . These species are mainly distinguished based on teleomorph morphology. Characteristics of anamorphic state are limited to allow their identification. ITS sequences for E. baeumleri, E. pisi and E.viciae–unijugae are available in GenBank and are well–distinguished from Vicia/Sesbania powdery mildew sequences obtained in this study. We could not find ITS sequence for E. ludens but anamorph for this species was not recognized and the fungus is endemic to Canada. We found three substitutions between E. trifoliorum (from Iran) and Vicia/Sesbania powdery mildew fungus. However, several ITS sequences under the name of E. trifoliorum were found to be 100% similar to Vicia/Sesbania powdery mildew. A group of species including E. trifoliorum, E. sesbaniae, E. robiniae, E. sophorae, E. crispula, all on Fabaceae, are morphologically closely related and make a complex species with strongly sinuous–subgeniculate, thick–walled chasmothecial appendages (see Braun & Cook 2012). E. sesbaniae is recently described (Braun et al. 2010). Before description of E. sesbaniae, one record of E. trifoliorum on Sesbania belongs to Ukraine (Dudka et al. 2004).

Without ITS or other gene sequences of the holotype of E. sesbaniae, it is impossible to make conclusion whether our new ITS sequence on Vicia faba actually belongs to E. sesbaniae or E. trifoliorum complex. However, this fungus on Sesbania punicea has already been recorded under the name of E. sesbaniae on Sesbania punicea from Iran (Abbasi et al. 2013, Sharifi et al. 2013). This is the first record of E. trifoliorum complex on Vicia faba. Moreover, this is the first time that rDNA ITS sequences is recorded for Erysiphe on Sesbania.

Erysiphe on Lagerstroemia indica

Two powdery mildews belonging to the genus Erysiphe viz. Erysiphe australiana (McAlpine) U. Braun & S. Takam. and Pseudoidium. yenii (U. Braun) U. Braun & R.T.A. Cook have been recorded on Lagerstroemia spp. Both species have previously been recorded from Iran (Abbasi et al. 2013, Sharifi et al. 2013). Both reports are based on anamorphic state from Guilan province and teleomorph has not been recognized.  Braun & Cook (2012) stated that relation of Ps. yenii to E. australiana is not clear, however, Ps. yenii differs by having narrower hyphae, conidiophores and type of conidial germination (long germination tube without lobed appressoria). This collection in Guilan province possesses wide hyphae and short germination tube with lobed appressoria and belongs to E. australiana. Sequence of ITS regions from this fungus showed 100% similarity to E. australiana. We could not demonstrate presence of Ps. yenii by molecular or morphological data. This is the first record of ITS sequence for E. australiana from Iran.

Erysiphe on Platanus orientalis

Erysiphe platani (Howe) U. Braun & S. Takam. is a well–known fungus occurring on Platanus orientalis worldwide. This species restricted to species of Platanus. Despite the fact that the anamorph state of E. platani occurs all over Guilan province and some other regions of Iran such as Tehran, Gorgan, Mazandaran provinces (Ershad 1995, Khodaparast & Abbasi 2009), however, there is no report for teleomorph of the fungus. All reports are based on anamorphic state. We sequenced full ITS region except for first 11 nucleotides that were removed due to ambiguous reading. This sequence is 100% similar to more than 10 sequences available in GenBank (NCBI). This is the first attempt for the identification of E. platani using rDNA sequence analysis in Iran.

Fig. 1. A Minimum Evolution tree based on ITS sequences for 20 taxa of Erysiphe species. The optimal tree with the sum of branch length = 0.32298257 is shown. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. All positions containing gaps and missing data were eliminated. There were a total of 585 positions in the final dataset. Evolutionary analyses were conducted in MEGA 7.

Fig. 2. A Minimum Evolution tree based on ITS sequences for 43 taxa of powdery mildews including Podosphaera and Golovinomyces species. The optimal tree with the sum of branch length = 0.29079068 is shown. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. All positions containing gaps and missing data were eliminated. There were a total of 413 positions in the final dataset. Evolutionary analyses were conducted in MEGA 7.

Podosphaera on Vigna

Heavily infected plants were observed in the greenhouse. Conidium morphology of the fungus more or less agrees with P. xanthii (Castagne) U. Braun & Shishkoff, however, accurate detection of P. xanthii from closely related species such as P. fusca based solely on conidium morphology is not possible. Sequence analysis showed that Vigna powdery mildew fungus in Iran is clustered with taxa belonging to P. xanthii complex.  Anamorphic state of P. xanthii largely agrees with those of some related species such as P. fusca (Fr.) U. Braun & Shishkoff and P. erigerontis–canadensis (Lév.) U. Braun & T.Z. Liu, however, teleomorph is distinguished by having larger ascomata and larger terminal oculi on asci (Braun & Cook 2012). Hirata et al. (2000), used two ITS sequences of Podosphaera from Vigna angularis (AB040297) and Vigna unguiculata (AB040340). According to this study Vigna powdery mildew isolates clustered in two haplotypes. The two haplotypes differ only at one position (442). Iranian isolate showed 100% similarity to sequence obtained from Podosphaera on Vigna angularis (AB04029) except for one non–identified position at base number 333 in this isolate. Recently, Ito & Takamatsu (2010) reconstructed a new phylogenetic tree of Podosphaera subsection Magnicellulatae based on 28S and ITS rDNA. They showed that Magnicellulatae taxa often infect the same plant genus or species. The most important host plants for P. xanthii complex in Iran include Cucumis sativa, Cucumis melo, Cucurbita spp. and Citrullus vulgaris. According to the previous phylogenetic studies (Hirata et al. 2000, Ito & Takamatsu 2010) and this study, Vigna isolates fell into phylogenetic group containing Cucurbitaceae host plants.

Podosphaera on Matricaria chamomilla

ITS sequence of chamomile (M. chamomilla) powdery mildew fungus showed 100% similarity to  P. xanthii on Xanthium strumarium (accession number: KX369236). In phylogenetic analysis this taxon fall on a clade containing ITS sequences from different host plant species including Xanthium, neotype genus for P. xanthii. According to Braun & Cook (2012) M. chamomilla is reported as a host plant for P. erigerontis–canadensis, but in our phylogenetic analysis this fungus showed close relationship to P. xanthii. There is one ITS sequence from Podosphaera on M. matricarioides (accession number: AB046988) in GenBank, but this sequence clustered with P. erigerontis–canadensis. As a result we conclude that M. chamomilla is infected with P. xanthii rather than P. erigerontis–canadensis in Guilan province.  

Golovinomyces on Dahlia

ITS sequence from Dahlia powdery mildew in Iran showed high similarity to Golovinomyces spadiceus (Berk. & M.A. Curtis) U. Braun from Zinnia elegans (100%, AB769425), Dahlia pinnata (100%, KX821733), Dahlia x cultorum (100%, AB077679), G. ambrosiae (Schwein.) U. Braun & R.T.A. Cook isolates from Ambrosia trifida (100%, AF011292), Helianthus tuberosus (100%, KY012249), Helianthus verticillatus (100%, KT310166), Helianthus annuus (100%, KM657962). Hence, ITS sequence might not possess enough variation to differentiate these two closely related species. G. spadiceus recently raised by Braun & Cook (2012). They pointed out some differences between the two species. According to these authors G. spadiceus differs by having narrower conidia and euoidium type of conidial germination. Morphological characterization of conidia and conidia germinations clearly showed that Dahlia powdery mildew is conspecific with G. spadiceus in Guilan province. Both species of Golovinomyces (G. ambrosiae and G. spadiceus) have been reported on Dahlia (Braun and Cook 2012).

Podosphaera on Epilobium

ITS sequence of Epilobium powdery mildew showed 99 % similarity (one base substitution) to Podosphaera epilobii (AB525926). Morphological characterization showed that this fungus could belong to P. epilobii.


The author would like to thank the two anonymous referees for their valuable comments that greatly improved the final version of the paper. This work was supported in part by a grant from the Deputy of Research and Technology of the University of Guilan, Iran.

Abbasi M, Boujari J, Donyadost–Chalan M. 2013. Notes on the powdery mildews (Erysiphaceae) in Iran. Iranian Journal of Plant Pathology 49: 345–49.
Boesewinkel HJ 1980. The morphology of the imperfect states of powdery mildews (Erysiphaceae). Botanical Review 46: 167–224. 
Braun U 1987. A monograph of the Erysiphales (powdery mildews). Nova Hedwigia 89: 1–700.
Braun U, Cook RTA 2012. Taxonomic manual of the Erysiphales (powdery mildews). CBS Biodivers Ser 11:1–707.
Braun U, Kruse J, Wolcan SM, Murace M. 2010. Three new species of the genus Erysiphe (Ascomycota, Erysiphales) on legumes and some new combinations. Mycotaxon112: 173–187.
Coyier DL. 1974. Heterothallism in the apple powdery mildew fungus, Podosphaera leucotricha. Phytopathology 64: 246–248.
Cunnington JH, Takamatsu S, Lawrie AC, Pascoe IG. 2003. Molecular identification of anamorphic powdery mildews (Erysiphales).Australasian Plant Pathology32: 421–428.
Dudka IO, Heluta VP, Tykhonenko YY, Andrianova TV, Hayova VP, Prydiuk MP, Dzhagan VV, Isikov VP.2004. Fungi of the Crimean Peninsula. M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Ukraine.
Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32:1792–1797.
Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783–791.
Khodaparast SA. 2007. A monograph on Erysiphaceae from Guilan province, Iran. University of Guilan press, Iran.
Khodaparast SA, Abbasi M. 2009. Species, host range, and geographical distribution of powdery mildew fungi in Iran. Mycotaxon 108: 213–216.
Khodaparast SA, Mohammadi AH, Haghdel M, Masigol H. 2016a. Taxonomy and phylogenetic position of Phyllactinia takamatsui, a newly described powdery mildew on cotoneaster, based on molecular and morphological data. Mycological Progress15: 1–5.
Khodaparast SA, Takamatsu S, Shadlou A, Damadi M, Pirnia M, Jahani M. 2016b. Notes on the genus Leveillula (Erysiphaceae): a new unrecorded species and notes on Leveillula infecting Ficus, Cucurbita and Tropaeolum in Iran. Phytotaxa 260: 267–275.
Khodaparast SA, Takamatsu S, Hedjaroude GA. 2001. Phylogenetic structure of the genus Leveillula (Erysiphales: Erysiphaceae) inferred from the sequences of the rDNA ITS regions with special references to the Leveillula taurica species complex. Mycological Research 105: 909–918.
Khodaparast SA, Niinomi S, Takamatsu S. 2007. Molecular and morphological characterization of Leveillula (Ascomycota: Erysiphales) on monocotyledonous plants. Mycological Research 111: 673–679.
Khodaparast SA, Takamatsu S, Harada M, Abbasi M, Samadi S. 2012. Additional rDNA ITS sequences and its phylogenetic consequences for the genus Leveillula with emphasis on conidium morphology. Mycological Progress 11: 741–752.
Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111–120.
Kumar S, Stecher G, & Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 3:1870–1874.
Hirata T, Takamatsu S. 1996. Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi. Mycoscience 37: 265–270.
Hirata T, Cunnington JH, Paksiri U, Limkaisang S, Shishkoff N, Grigaliunaite B, Sato Y, Takamatsu S. 2000. Evolutionary analysis of subsection Magnicellulatae of Podosphaera section Sphaerotheca (Erysiphales) based on the rDNA internal transcribed spacer sequences with special reference to host plants. Canadian Journal of Botany 78: 1521–1530.
Ito M, Takamatsu S. 2010. Molecular phylogeny and evolution of subsection Magnicellulatae (Erysiphaceae: Podosphaera) with special reference to host plants. Mycoscience 51: 34–43.
Nei M, Kumar S. 2000. Molecular evolution and phylogenetics. Oxford University Press, USA.
Rzhetsky A, Nei M. 1992. A simple method for estimating and testing minimum evolution trees. Molecular Biology and Evolution 9:945–967.
Saitou N, Nei M. 1987. The neighbor–joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425.
Sharifi K, Khodaparast SA, Mousanejzhad S. 2013. A contribution to the knowledge of taxonomy and identification of anamorphic genus Oidium in Guilan province, Iran. Iranian Journal of Plant Protection Science 44:1–13.
Shin HD. 2000. Erysiphaceae of Korea. Plant Pathogens of Korea 1:1–320.
Walsh PS, Metzger DA, Higuchi R. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR–based typing from forensic material. Biotechniques10: 506–513.
White CG 1970. Production of powdery mildew cleistocarps in a controlled environment. Transactions of the British Mycological Society 55: 355–365.
White TJ, Bruns TD, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal genes for phylogenetics. In: PCR Protocols: a guide to methods and applications (Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds): 315–322. Academic Press, USA.