Iranian Mycological Society Mycologia Iranica 2383-000X 5 2 2018 12 01 Enhancement of Xylanase Production by Protoplast Fusion of Trichoderma spp. 53 61 118826 10.22043/mi.2019.118826 EN Z. Papzan Department of Biotechnology, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran M. Javan-Nikkhah Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran M. Kowsari Microbial Biotechnology and Biosafety Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran A. Mirzadi Gohari Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran M. Carmen Limón Department of Genetics, Faculty of Biology, University of Seville, Seville, Spain Journal Article 2018 10 08 Filamentous fungi Trichoderma spp. are efficient hyper-producers of industrially important enzymes. Xylanases are essential hemicellulases having vast ranges of applications in the various industrial sectors. In this study, the protoplast fusion of two T. virens and T. harzianum strains resulted in 2.5 Uml-1 xylanase activity in fusant X3 showing 4.7-fold improvement in xylanase activity compared to that of parents with 0.54 Uml-1. Moreover, to evaluate the influence of protoplast fusion on the xylanase activity, the expression patterns of the xylanase gene xyn3 was analyzed in the parental strains and the using qPCR. The results demonstrated that the relative expression of the xyn3 increased in X3 by 4.9 fold compared to that of the parents. Finally, based on the results, it could be concluded that the protoplast fusion technology is a promising approach to generate new superior fungi with high production ability of the industrially important enzymes.

https://mij.areeo.ac.ir/article_118826_88dd5733c53d57452b7dffc73ed21472.pdf

Iranian Mycological Society Mycologia Iranica 2383-000X 5 2 2018 12 01 Molecular identification of Tilletia controversa and T. caries, the causal agent of wheat dwarf and common bunt 63 70 119652 10.22043/mi.2018.119652 EN B. Sharifnabi Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran F. Ghaderi Department of Plant Protection, College of Agriculture, Yasouj University, Iran F. Haghighi Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran Journal Article 2018 05 10 Common and dwarf bunt of wheat are recognized as being caused by three closely related species, Tilletia caries and T. laevis as common bunt, and T. controversa as dwarf bunt. The morphological characteristics of two species including T. controversa and T. caries were studied from wheat grown in two provinces of Iran, Lorestan and Chaharmahal–o-Bakhtiari during 2014-2015. In this study, morphological characters could not completely distinguish these pathogens, as in some properties, they showed similarity to T. laevis. So, a collection of twenty wheat bunt isolates was used to compare species in morphological characteristics and phylogenetic relationships. Phylogenetic analyses were presented based on three PCR amplified nuclear DNA fragments including elongation factor 1 alpha (EF1α), the second largest subunit of RNA polymerase II (RPB2) genes and ITS-rDNA region. Maximum likelihood (ML) method was used to determine the phylogenetic relationship among isolates using MEGA v.6 and BEAUti and BEAST v1.6.1 software. Maximum likelihood bootstrap (BS) values and Bayesian posterior probabilities (PP) values were applied as criteria for strongly supported clades. Two species including T. controversa and T. caries were distinguished as different species with DNA sequence information.

https://mij.areeo.ac.ir/article_119652_f5724cbe1f35362e47edc3b07930d072.pdf

Iranian Mycological Society Mycologia Iranica 2383-000X 5 2 2018 12 01 Polyphasic identification of Sepedonium microspermum isolated from two genera of Boletales in Iran 71 77 119640 10.22043/mi.2018.119640 EN M. Torbati Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran M. Arzanlou Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran A. Babai-Ahari Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Journal Article 2018 07 03 Moldy Boletus sp. and Xerocomus sp. were collected from several locations at the campus of the University of Tabriz, Iran. Fungicolous fungal isolates were recovered and characterized by the combination of morphological traits and phylogenetic analyses of combined ITS and LSU sequence data. Fungal isolates were identified as Sepedonium microspermum. This is the first report of S. microspermum on Xerocomus sp. from Iran which is comprehensively described and illustrated.

https://mij.areeo.ac.ir/article_119640_f082feba99a336a718b6b907ff9f6d17.pdf

Iranian Mycological Society Mycologia Iranica 2383-000X 5 2 2018 12 01 Notes on Dictyuchus species (Stramenopila, Oomycetes) from Anzali lagoon, Iran 79 89 120353 10.22043/mi.2018.120353 EN H. Masigol Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran S.A. Khodaparast Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran R. Mostowfizadeh-Ghalamfarsa Department of Plant Protection, Faculty of Agriculture, University of Shiraz, Shiraz, Iran S. Mousanejad Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran K. Rojas-Jimenez School of Biology, University of Costa Rica, San José, Costa Rica H. P. Grossart Department of Experimental Limnology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Stechlin, Germany Journal Article 2018 06 06 Nine Dictyuchus isolates were obtained from floating and decaying twigs and leaves in Anzali lagoon, Anzali County, Guilan province, Iran. They are interestingly distinguished from current Dictyuchus species by the presence of dictyoid and achlyoid type of zoospore discharge, abundant fusiform and spherical gemma, and the absence of any sexual apparatus. In addition, phylogenetic analyses of the internal transcribed spacer (ITS) region of the nuclear rDNA and cytochrome c oxidase subunit I (cox1) gene sequences using Maximum likelihood method indicate its novelty. We preferred not to introduce the isolates as new species due to low number of and sometimes unreliable sequences and lack of type species and suggested to verify Dictyuchus sterilis which has been excluded before. We discussed about taxonomy of the genus in details and provided a revised key to the species. In addition, it was shown that sterility in vitro might be common in this genus.

https://mij.areeo.ac.ir/article_120353_602ebc1de1ff48de0c42a5422e4d087c.pdf

Iranian Mycological Society Mycologia Iranica 2383-000X 5 2 2018 12 01 Fusarium species associated with medicinal plants of Lamiaceae and Asteraceae 91 101 120383 10.22043/mi.2019.120383 EN A. Habibi Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran S. M. Mansouri Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran B. Sadeghi Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran Journal Article 2018 08 17 The purpose of this study was to identify the Fusarium species associated with selected medicinal plant species of Lamiaceae and Asteraceae in Kerman, the second-largest province of Iran. The plants showing wilting/rotting symptoms as well as asymptomatic plants were collected from main medicinal plants reservoirs and production areas. Fusarium species were identified based on morphological characteristics as well as subsequent sequencing of the translation elongation factor 1-alpha (TEF1-α gene. The following species were identified: F. oxysporum, F. solani and F. nygamai from Achillea wilhelmsii (Asteraceae); F. solani, F. proliferatum and species of F. tricinctum species complex from Teucrium polium (Lamiaceae); F. solani, F. proliferatum and species of F. tricinctum species complex from Ziziphora tenuior (Lamiaceae); F. solani and a species of F. tricinctum species complex from Thymus carmanicus (Lamiaceae); F. redolens, F. brachygibbosum, F. proliferatum, F. tricinctum species complex, F. compactum and F. equiseti from Mentha piperita (Lamiaceae); and F. solani and F. proliferatum from Ziziphora cliniopodiodes (Lamiaceae). For the first time, the pathogenicity of Fusarium nygamai on Achillea wilhelmsii as well as F. redolens and F. brachygibbosum on Mentha piperita were confirmed in this study.

https://mij.areeo.ac.ir/article_120383_dbc6fb422efeab721220f4028ad3de14.pdf

Iranian Mycological Society Mycologia Iranica 2383-000X 5 2 2018 12 01 First report of Rhytidhysteron hysterinum from Iran 103 104 118828 10.22043/mi.2018.118828 EN S. A. Mousavi Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran S. A. Khodaparast Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran S. Mousanejad Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran A. Rouhibakhsh Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran Journal Article 2018 10 10 ----- -----

https://mij.areeo.ac.ir/article_118828_4c989d27ce8fc264091eb5bea2aba79f.pdf

Iranian Mycological Society Mycologia Iranica 2383-000X 5 2 2018 12 01 Septoria malagutii as an endophytic fungus of Achillea millefolium from Iran 105 107 120384 10.22043/mi.2018.120384 EN S. Hatamzadeh Department of Plant Protection, Faculty of crop Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran K. Rahnama Department of Plant Protection, Faculty of Crop Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran S. Nasrollanejad Department of Plant Protection, Faculty of crop Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Kh-B. Fotouhifar Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran Kh. Hemmati Department of Plant Protection, Faculty of Crop Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran J. White Department of Plant Biology, Rutgers University, New Brunswick, New Jersey, USA Journal Article 2018 06 26 ---- ----

https://mij.areeo.ac.ir/article_120384_a478768c44bceacfd50bb6929c496aca.pdf