Iranian Mycological Society
Mycologia Iranica
2382-9664
2382-9656
3
2
2016
12
01
New reports of endophytic fungi associated with cherry (Prunus avium) and sour cherry (Prunus cerasus) trees in Iran
75
85
EN
Sh.
Abdollahi Aghdam
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
abdollahi_aghdam@yahoo.com
Kh.–B.
Fotouhifar
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
fotowhi@ut.ac.ir
10.22043/mi.2017.112557
Endophytic fungi constitute a remarkable multifarious group of microorganisms live within plants tissues without causing any obvious negative effect. Endophytic fungi have been found in every plant species examined to date. During an investigation on endophytic fungi, 123 fungal isolates were obtained from healthy twigs and leaves of cherry (<em>Prunus avium</em>) and sour cherry (<em>P.</em> <em>cerasus</em>) trees in Iran. The isolates identified based on sequence data of 18S rDNA (SSU) region, as well as morphological and cultural features. As a result, four species namely <em>Coniothyrium olivaceum</em>, <em>Collophora paarla</em>, <em>Sarocladium strictum</em> and <em>Quambalaria cyanescens</em> identified. All these identified species are new reports as endophytic fungi from <em>P. cerasus</em> and <em>P</em>. <em>avium</em> in the world. Among them, <em>Collophora paarla</em> and <em>Quambalaria cyanescens</em> are new taxa for the mycobiota of Iran.
diversity,Morphology,taxon,Sequencing,phylogeny
https://mij.areeo.ac.ir/article_112557.html
https://mij.areeo.ac.ir/article_112557_9c5d76c47b5f2dd1168dc3df95b654cf.pdf
Iranian Mycological Society
Mycologia Iranica
2382-9664
2382-9656
3
2
2016
12
01
Identification of yeast species from uncultivated soils by sequence analysis of the hypervariable D1/D2 domain of LSU–rDNA gene in Kermanshah province, Iran
87
98
EN
S.
Jamali
https://orcid.org/00
Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
jamali454@yahoo.com
M.
Gharaei
Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
S.
Abbasi
Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
10.22043/mi.2017.26130
Yeasts are a polyphyletic group of ascomycete and basidiomycete fungi characterized by having a unicellular growth phase and sexual stages that are not enclosed in fruiting bodies. An attempt was made to identify yeast species in uncultivated soils collected from different areas of Kermanshah province, Iran, by analyzing hypervariable D1/D2 domain of the large subunit (LSU) rDNA gene sequenceand comparing the sequences with that available in NCBI database. In this study, 25 soil samples were analyzed and eight species including <em>Rhodotorula</em> <em>toruloides </em>(KP324973)<em>*,</em> <em>Trichosporon</em> <em>coremiiforme</em> (KP055040)<em>*</em>, <em>Naganishia</em> <em>uzbekistanensis </em>(KP324959)<em>, Candida catenulata</em> (KP324968), <em>C. paracilopsis</em> (KP324965)<em>*</em>, <em>C. boidinii</em> (KP324 962)<em>*</em>, <em>Lecythophora</em> sp. (KP336745)<em>*</em> and <em>Meyerozyma guilliermondii </em>(KPKP324971, KP324978)were identified. Phylogenetic analysis based on D1/D2 regions allowed us to establish the precise taxonomic placement of each species. The Canonical correspondence analysis (CCA) indicated that elevation, EC, pH, and clay were important environmental parameters influencing basidiomycete yeast distribution in uncultivated soils. In this study, the presence of eight species is confirmed that asterisk species are the new records for the mycobiota of Iran.
Yeasts,rDNA genes,phylogeny,Canonical Correspondence Analysis
https://mij.areeo.ac.ir/article_26130.html
https://mij.areeo.ac.ir/article_26130_c911e1fb9de821b053ba154370c84af2.pdf
Iranian Mycological Society
Mycologia Iranica
2382-9664
2382-9656
3
2
2016
12
01
A taxonomic study on Stemphylium species associated with black (sooty) head mold of wheat and barley in Iran
99
109
EN
A.
Poursafar
0000-0002-4296-8197
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
a.rezapoursafar@gmail.com
Y.
Ghosta
Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran
ghoosta@gmail.com
M.
Javan-Nikkhah
0000-0001-9533-0157
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
jnikkhah@ut.ac.ir
10.22043/mi.2017.26183
<em>Stemphylium</em> as a monophyletic genus of filamentous ascomycetes, comprises both of saprophytic and plant pathogenic species with worldwide distribution. In an investigation of fungi associated with the black (sooty) head mold of wheat and barley in different regions of Golestan, Alborz and Qazvin provinces, thirty–two isolates with typical characteristics of the genus <em>Stemphylium</em> were recovered. All isolates were subjected to morphological assessments and DNA sequence analyses (ITS–rDNA and a part of <em>GPDH </em>gene). As a result, four species <em>viz</em>. <em>Stemphylium alfalfae</em>, <em>S. eturmiunum</em>, <em>S. lycii</em> and <em>S. vesicarium </em>were identified. The association of all identified species with the black head mold symptoms of wheat and barley is reported for the first time and <em>S. eturmiunum</em> and <em>S. lycii</em> are new records to the mycobiota of Iran.
disease,Morphology,DNA analysis,phylogeny,Taxonomy
https://mij.areeo.ac.ir/article_26183.html
https://mij.areeo.ac.ir/article_26183_058b1faba8ca8cd60b17f722e976bb22.pdf
Iranian Mycological Society
Mycologia Iranica
2382-9664
2382-9656
3
2
2016
12
01
Genetic variability of Paecilomyces variotii isolates, the causal agent of die–back disease in pistachio, using ITS–RFLP analysis
111
120
EN
S. K.
Sabbagh
Department of Biology, College of Science, Yazd University, Yazd, Iran
sksabbagh@yazd.ac.ir
F.
Khosravi Moghaddam
Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran
fatemeh.khosravi_66@yahoo.com
10.22043/mi.2016.112566
<em>Paecilomyces variotii</em> is one of the most important causal agents of dieback disease in pistachio (<em>Pistacia vera</em>) gardens. The disease affects different parts of the tree, such as branch and trunk. Assessment of genetic structure in different populations of this species will lead to more useful management of pistachio dieback disease. In this study, genetic variation within samples of <em>P</em><em>. variotii </em>isolates from different geo-climatic origins of Kerman province was studied using ITS- RFLP analysis. Universal primer pairs AB28 and TW81 were used for ITS region amplification. Thirteen restriction enzymes were subjected to digest PCR products. Seven out of the 13 restriction enzyme including: EcoR І, Hpyf 3І, Apa І, Hinf І, Mbo І, Msp І, Rsa І showed restriction pattern. Jaccard's similarity coefficient used to determine of genetics similarity and cluster analysis dendrog was designed by using UPGMA algorithm method. Data analysis showed a high similarity at the level of 70% between isolates and all isolates were divided into 9 distinct groups. Analysis of molecular variance (AMOVA) showed a variation of 85% and 15% among of within isolates, respectively. Based on these results we can conclude that ITS-RFLP is useful for wider genetic diversity assessment and epidemiological studies of distantly related isolates. The future studies could be performed to develop new molecular markers to detect this fungus in field.
Phytopathogen,population,restriction enzyme,molecular marker
https://mij.areeo.ac.ir/article_112566.html
https://mij.areeo.ac.ir/article_112566_ce607fd7690fac699e072573ca0c4cd3.pdf
Iranian Mycological Society
Mycologia Iranica
2382-9664
2382-9656
3
2
2016
12
01
Detection of the fumonisin-producing Fusarium fujikuroi species complex (FFSC) associated with wild grasses in Iran
121
126
EN
Kh.
Chehri
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
khchehri@gmail.com
10.22043/mi.2016.112592
In order to determine the fumonisin producingisolates of the <em>Fusarium fujikuroi </em>species complex (FFSC), associated with infected spikes, and ear rot of grass plants, we examined 78 samples collected from Kermanshah province, Iran. Based on morphological characters, twenty two isolates assigned to the FFSC and were identified as <em>F. proliferatum </em>(ten isolates), <em>F. verticillioides </em>(seven isolates)<em>, </em>and <em>F. subglutinans</em> (five isolates). This was additionally approved using the specific primers of <em>F. verticillioides, F. proliferatum</em>, and <em>F. subglutinans</em>, i.e. VER1/VER2, PRO1/PRO2, and SUB1/SUB2, respectively. PCR-based detection of the fumonisin-producing isolates of FFSC was also achieved using the primers FUM1 F/FUM1 R. Out of twenty two isolates, eleven isolates (50%) determined as fumonisin chemotype.
Fusarium spp,grasses,molecular detection,Mycotoxins,Iran
https://mij.areeo.ac.ir/article_112592.html
https://mij.areeo.ac.ir/article_112592_2b54e11454205ff28fa0fdd95c0b4199.pdf
Iranian Mycological Society
Mycologia Iranica
2382-9664
2382-9656
3
2
2016
12
01
Molecular identification of some anamorphic powdery mildews (Erysiphales) in Guilan province, north of Iran
127
133
EN
S. A.
Khodaparast
0000-0002-7480-4170
Department of Plant Protection, College of Agricultural Sciences, University of Guilan, Rasht
khodaparast@guilan.ac.ir
10.22043/mi.2017.68336.1095
In this study, ITS–rDNA region was used to identify some anamorphic powdery mildews in Guilan province. According to the results, <em>Erysiphe</em> species on <em>Vicia faba</em> and <em>Sesbania</em> <em>punicea</em> showed 100% similarity to each other, however, without ITS sequence of holotype of <em>E. sesbaniae</em> it is impossible to make conclusion whether <em>Vicia faba</em> powdery mildew fungus actually belongs to <em>E. sesbaniae</em> or <em>E. trifoliorum</em> complex. ITS sequence from isolate of <em>Lagerstroemia indica</em> powdery mildew showed 100% similarity to <em>E. australiana. Podosphaera</em> on <em>Vigna</em> fells into phylogenetic group containing <em>P. xanthii</em> on cucurbitaceous hosts. ITS sequence of chamomile (<em>Matricaria chamomilla</em>) powdery mildew fungus showed 100% similarity to <em>P. xanthii</em> on <em>Xanthium strumarium. </em>Molecular characteristics and morphological examination of conidia and conidia germination clearly showed that <em>Dahlia</em> powdery mildew in Guilan province is conspecific with <em>G. spadiceus</em>. Anamorph morphology and ITS sequence of <em>Podosphaera</em> on <em>Epilobium</em> and <em>Erysiphe</em> on <em>Platanus</em> <em>orientalis</em> showed that these species belong to <em>P. epilobii</em> and <em>Erysiphe platani </em>respectively<em>.</em>
Erysiphaceae,Erysiphe,Oidium,Podosphaera,Pseudoidium
https://mij.areeo.ac.ir/article_113055.html
https://mij.areeo.ac.ir/article_113055_77f0a4039d3fb384b9235527a6a0c7d3.pdf
Iranian Mycological Society
Mycologia Iranica
2382-9664
2382-9656
3
2
2016
12
01
Two new hyphomycete species from petroleum-contaminated soils for mycobiota of Iran
135
140
EN
E.
Mohammadian
Department of Plant Protection, University of Tabriz, Tabriz, Iran
elmo_elham@yahoo.com
M.
Arzanlou
Department of Plant Protection, University of Tabriz, Tabriz, Iran
arzanlou@tabrizu.ac.ir
A.
Babai-Ahari
Department of Plant Protection, University of Tabriz, Tabriz, Iran
ababaiahari@yahoo.com
10.22043/mi.2016.25918
During a study on fungal diversity in petroleum contaminated soils in Khuzestan province, two species of hyphomycetes were isolated, which represent new records for the mycobiota of Iran. Based on the combination of morphological data and ITS-rDNA sequence, the isolates were identified as Alternaria obovoidea and Emericellopsis pallida. Morphological descriptions and illustrations are provided for the species. To our knowledge, this is the first report of these two species from petroleum contaminated soils in the world.
Petroleum pollution,fungal diversity,ITS-rDNA,new record
https://mij.areeo.ac.ir/article_25918.html
https://mij.areeo.ac.ir/article_25918_1768688e6053aee481932acf3e43c659.pdf