Iranian Mycological SocietyMycologia Iranica2382-96645120180601Notes on the genus Pileolaria (Pucciniales)1611746810.22043/mi.2018.117468ENM. AbbasiDepartment of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, IranJournal Article20180212The results showed that <em>Pileolaria terebinthi</em>, type species of the genus <em>Pileolaria</em> is not a hemi–form or brachy–form rust species as mentioned among literature by several authors. Study on miscellaneous specimens collected from Iran and locus classicus of the species, France, revealed that <em>P. terebinthi</em> is an automacrocyclic rust and its aecial state is not uredinioid as formerly believed. This species has caeoma–type aecial state which differs from its Uredo–type uredinial state by having different spore ornamentation and ontogeny. The genus <em>Pileolaria</em> is redefined on the basis of these new findings. Moreover, all spore states of <em>P. terebinthi</em>, are documented using microscopy and photomicrography. New information about host range and distribution of <em>P. terebinthi</em> in Iran is also provided.Iranian Mycological SocietyMycologia Iranica2382-96645120180601Biocontrol activity of endophytic fungus of barley, Microdochium bolleyi, against Gaeumannomyces graminis var. tritici71411820510.22043/mi.2019.118205ENL. ShadmaniDepartment of Plant Protection, College of Agriculture, Razi University, Kermanshah, IranS. JamaliDepartment of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iranhttps://orcid.org/00A. FatemiDepartment of Soil Science, College of Agriculture, Razi University, Kermanshah, IranJournal Article20170107In this study, two isolates of genus <em>Microdochium</em> (W2 and B26) was isolated from the roots of healthy barley plants in agricultural fields from Kermanshah province in 2014 and were identified as <em>Microdochium bolleyi</em> based on morphological and molecular characteristics. Dual culture studies revealed that W2 and B26 inhibit 45% and 20% of the radial growth of <em>Ggt </em>in turn. The W2 isolate was inoculated on barley roots in order to assess its effect on suppressing take–all disease and promoting the growth of barley plants. Regarding suppression of disease test, pathogenicity index (the percentage of necrosis root disease severity) for the plants that were inoculated with endophytic <em>M. bolleyi</em> and <em>Ggt</em> at the same time was 0.6, compared to 4.4 for the plants that were inoculated with <em>Ggt</em> alone. <em>M. bolleyi</em> also increased significantly root fresh weight by 31.21%, aerial fresh weight by 15.15%, root length by 3.0%, aerial length by 2.35%, root dry weight by 30.94% and aerial dry weight by 12.28% which were significant differences at the 5% level. For growth-promoting effects, growth parameters were evaluated and the results showed <em>M. bolleyi</em> effectively promoted root fresh weight by 60.0%, aerial fresh weight by 38.46%, root length by 4.54%, aerial length by 7.21%, root dry weight by 60.43% and aerial dry weight by 38.60%, which were significant in 5% level. To our knowledge, this is the first report of <em>M. bolleyi</em> for the mycobiota of Iran and it may be further used as a biocontrol agent.Iranian Mycological SocietyMycologia Iranica2382-96645120180601Morphological and molecular characterization of Oomycetes associated with root and crown rot of cucurbits in Kermanshah province, Iran152711840910.22043/mi.2019.118409ENA. Hosseini BadrbaniDepartment of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, IranS. AbbasiDepartment of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, IranZ. BolboliDepartment of Plant Protection, School of Agriculture, Shiraz University, Shiraz, IranS. JamaliDepartment of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iranhttps://orcid.org/00R. SharifiDepartment of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, IranJournal Article20180201<em>Pythium</em> and <em>Phytophthora</em> are among the most well-known plant pathogens around the world that cause rotting of seeds, root, and crown, seedling death, and soft rot of fruits in contact with the soil. In this research, 347 isolates of these two genera and their close genus, <em>Phytopythium</em> were isolated from the cucurbits fields in Kermanshah province, Iran and examined in terms of morphological and physiological characteristics. ITS-rDNA region and the partial cytochrome oxidase II (<em>cox</em> II) gene from the selected isolates were amplified and sequenced to confirm the morphological identification. Based on the morphological, morphometrical, physiological, and phylogenetic examinations, nine species of <em>Pythium</em> including<em> P. aphanidermatum</em>, <em>P. dissotocum</em>, <em>P. catenulatum</em>, <em>P. kashmirense</em>, <em>P. middletonii</em>, <em>P. nodosum</em>, <em>P. oligandrum</em>, <em>P. torulosum,</em> and <em>P. ultimum</em>; two species of <em>Phytopythium </em>including <em>Pp. mercuriale</em> and <em>Pp. litorale</em>, and three species of <em>Phytophthora</em> including <em>Ph. melonis</em>,<em> Ph. nicotianae,</em> and <em>Ph. parasitica</em> were detected. Among the species identified in this study, <em>Pp. mercuriale</em> was a new record for mycobiota of Iran and two species, <em>P. aphanidermatum </em>and <em>P. ultimum </em>were isolated more frequently.<br /> Iranian Mycological SocietyMycologia Iranica2382-96645120180601Colletotrichum gloeosporioides sensu stricto, the causal agent of a leaf spot disease of Schefflera arboricola in Iran293411840410.22043/mi.2019.118404ENS. BagherabadiDepartment of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, IranD. ZafariDepartment of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, IranF. Ghobadi AnvarDepartment of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, IranU. DammSenckenberg Museum of Natural History Görlitz, PF 300 154, 02806 Görlitz, GermanyJournal Article20180210<em>Schefflera arboricola</em> is a flowering plant in the family <em>Araliaceae</em>; its common name is dwarf umbrella tree. It is an evergreen shrub and a popular ornamental plant, commonly grown as an indoor plant. During a sampling in 2015, a new leaf spot symptoms was observed on <em>S. arboricola </em>plants in several greenhouses in the Hamadan province, Iran. Most of the plants were severely damaged by this disease. The presumed causal agent was isolated from symptomatic leaves. Based on morphological and cultural characteristics, the fungus was identified as a <em>Colletotrichum</em> species, probably belonging to the <em>C. gloeosporioides </em>species complex. By means of molecular data (<em>TUB2</em> & <em>GAPDH</em>) the fungus was revealed to be <em>C. gloeosporioides</em>sensu stricto. Pathogenicity tests showed that the fungus is the causal agent of leaf spot on <em>S. arboricola</em> shrubs. To our knowledge, this is the first report of <em>C. gloeosporioides </em>sensu stricto on <em>S. arboricola</em> in Iran.<br /> <em> </em>Iranian Mycological SocietyMycologia Iranica2382-96645120180601Identification of the species Parastagonospora dactylidis on poaceous plants in Iran354111807510.22043/mi.2019.118075ENF. GhaderiDepartment of Plant Protection, College of Agriculture, Yasouj University, Iranhttps://orcid.org/00M. RazaviPlant Pathology Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, IrannullJournal Article20180210The purpose of this study was to identify new species of <em>Parastagonospora</em> in association with poaceous plants in Iran. Symptomatic leaves and ears were collected from different poaceous species in the field from provinces including Fars, Khuzestan and Kohgiluyeh and Boyerahmad. In the present research, 12 isolates based on morphological characteristics and sequencing of LSU and ITS-rDNA regions were recognized as <em>P.</em> <em>dactylidis</em> on<em> Phalaris arundinacea </em>(fiveisolates), <em>Bromus hordeaceus</em> (three isolates) and<em> Aegilops tauschii </em>(four isolates). The species of <em>P.</em> <em>dactylidis</em> is reported from Iran for the first time and also all of the identified hosts are new host to the world.Iranian Mycological SocietyMycologia Iranica2382-96645120180601Clonostachys rosea, a new and promising entomopathogenic fungus infecting pupa of jujube fruit fly, Carpomya vesuviana434911875310.22043/mi.2019.118753ENH. MahmoudiPlant Protection Research Department, South Khorasan Agricultural and Natural Resources Research and Education Center, AREEO, Birjand, IranA. AminiDepartment of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, IranM. R. MirzaeePlant Protection Research Department, South Khorasan Agricultural and Natural Resources Research and Education Center, AREEO, Birjand, IranH. SadeghiDepartment of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, IranG. R. TavakkoliPlant Protection Research Department, South Khorasan Agricultural and Natural Resources Research and Education Center, AREEO, Birjand, IranJournal Article20170520Jujube fruit fly, <em>Carpomyia vesuviana</em>, is the most important pest of jujube in Iran. During February and March 2013, a survey on fungi associated with pupae of <em>C</em><em>. vesuviana </em>was conducted in Birjand, South Khorasan province of Iran. To isolate fungi, the samples were washed under running tap water, surface-sterilized in 0.5% sodium hypochlorite, then rinsed and placed onto potato dextrose agar. Out of eight isolates of <em>Clonostachys rosea</em> obtained in this study, only one showed considerable pathogenicity against the pupa of <em>C</em><em>. vesuviana</em>. This isolate was additionally examined using the morphological and molecular studies, based on ITS-rDNA. Both morphological and molecular data confirmed the identity of this fungus as <em>Clonostachys rosea</em>. Based on pathogenicity assays, significant mortality of inoculated pupa (46%) was recorded with a conidial suspension of 10<sup>10</sup> (spore/ ml). Median Lethal Time (LT<sub>50</sub>) and LC50 values of this isolate against pupa of<em> C. vesuviana</em> were calculated 4.6 days and 5.1×10<sup>4</sup> spore/ml, respectively. A significant positive correlation between different 3<em> C. rosea</em> concentrations and mortality of <em>C. vesuviana</em> was observed (r<sup>2</sup>= 0.9986, p= 0.003). <em>Clonostachys rosea</em> is here reported as a newand promising entomopathogenic fungus on <em>C. vesuviana</em>, suggesting that it is likely to play roles in protecting jujube trees against this pest.Iranian Mycological SocietyMycologia Iranica2382-96645120180601Misidentification of Urocystis species on Ceratocephala falcata (Ranunculaceae) from Northern Iran515211773010.22043/mi.2018.121288.1110ENM. AbbasiDepartment of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, IranJournal Article20180404----------------
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