TY - JOUR ID - 24665 TI - Scanning Electron Microscopy (SEM) analysis and biological control of Ixodes ricinus using entomopathogenic fungi JO - Mycologia Iranica JA - MI LA - en SN - 2382-9664 AU - Pirali-Kheirabadi, KH. AU - Razzaghi-Abyaneh, M. AU - Eslamifar, A. AU - Halajian, A. AU - Nabian, S. AD - Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran AD - Department of Mycology, Pasteur Institute of Iran, Tehran, Iran AD - Department of Pathology, Pasteur Institute of Iran, Tehran, Iran AD - Department of Biodiversity (Zoology), University of Limpopo, Sovenga, South Africa AD - Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Y1 - 2016 PY - 2016 VL - 3 IS - 1 SP - 39 EP - 46 KW - biological control KW - Ixodes ricinus KW - Metarhizium anisopliae KW - Enthomopathogenic fungi DO - 10.22043/mi.2017.41288.1065 N2 - In the present study, pathogenicity of four native strains of Entomopathogenic fungus; Metarhizium anisopliae, was studied against adult stage of Ixodes ricinus. For this purpose a total number of 180 adult ticks were examined in triplicate. Thirty ticks for each strain and negative and positive controls were immersed in 2.4×107 fungal conidia/ml in vitro. Samples were incubated in separate Petri dishes at 26 °C and 70% relative humidity. Positive control groups were treated with Cypermethrin and negative controls were immersed in the same volume of sterilized distilled water. Mortality rate and fungal grow on ticks for each strain were reported in comparison with control groups. M. anisopliae IRAN 437 C showed the highest virulence in mortality and mycelium grow on ticks.. Cypermethrin killed the ticks with higher potency than that of entomopathogenic fungi. Scanning electron microscopy showed the growth of M. anisopliae on the surface of tick bodies and penetration of fungal hyphae through tick cuticle. Taken together, results obtained from this study show potential of Iranian Entomopathogenic fungi as an biocontrol agents of I. ricinus. This is the first report demonstrates the mechanism of action of entomopathogenic fungi of the genus Metarhizium on ticks at electron microscopy level. UR - https://mij.areeo.ac.ir/article_24665.html L1 - https://mij.areeo.ac.ir/article_24665_2d67fb573a8a8190e0017b3dc50fcefb.pdf ER -