TY - JOUR ID - 112566 TI - Genetic variability of Paecilomyces variotii isolates, the causal agent of die–back disease in pistachio, using ITS–RFLP analysis JO - Mycologia Iranica JA - MI LA - en SN - 2382-9664 AU - Sabbagh, S. K. AU - Khosravi Moghaddam, F. AD - Department of Biology, College of Science, Yazd University, Yazd, Iran AD - Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran Y1 - 2016 PY - 2016 VL - 3 IS - 2 SP - 111 EP - 120 KW - Phytopathogen KW - population KW - restriction enzyme KW - molecular marker DO - 10.22043/mi.2016.112566 N2 - Paecilomyces variotii is one of the most important causal agents of dieback disease in pistachio (Pistacia vera) gardens. The disease affects different parts of the tree, such as branch and trunk. Assessment of genetic structure in different populations of this species will lead to more useful management of pistachio dieback disease. In this study, genetic variation within samples of P. variotii isolates from different geo-climatic origins of Kerman province was studied using ITS- RFLP analysis. Universal primer pairs AB28 and TW81 were used for ITS region amplification. Thirteen restriction enzymes were subjected to digest PCR products. Seven out of the 13 restriction enzyme including: EcoR І, Hpyf 3І, Apa І, Hinf І, Mbo І, Msp І, Rsa І showed restriction pattern. Jaccard's similarity coefficient used to determine of genetics similarity and cluster analysis dendrog was designed by using UPGMA algorithm method. Data analysis showed a high similarity at the level of 70% between isolates and all isolates were divided into 9 distinct groups. Analysis of molecular variance (AMOVA) showed a variation of 85% and 15% among of within isolates, respectively. Based on these results we can conclude that ITS-RFLP is useful for wider genetic diversity assessment and epidemiological studies of distantly related isolates. The future studies could be performed to develop new molecular markers to detect this fungus in field. UR - https://mij.areeo.ac.ir/article_112566.html L1 - https://mij.areeo.ac.ir/article_112566_ce607fd7690fac699e072573ca0c4cd3.pdf ER -